| Project/Area Number |
24560052
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied optics/Quantum optical engineering
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
SUDA AKIRA 東京理科大学, 理工学部, 教授 (80250108)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAHASHI Hiroshi 東京理科大学, 理工学部, 助教 (70572709)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 蛍光タンパク質 / 非線形分光 / 光褪色 / 二光子励起 / バイオイメージング / 非線形光学 / 分光 |
|---|
| Outline of Final Research Achievements |
Based on the measurement of the photobleaching action spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy, we found that the photobleaching occurs through excited-state absorption following two-photon excitation from the ground state. An unidentified dark state with a longer lifetime may be the initial state for excited-state absorption besides the triplet excited state. Then, the adaptive control method was applied to modulate the spectral amplitude and phase of excitation pulses maximizing the two-photon excited fluorescence while suppressing the photobleaching. As a result, the spectral amplitude was optimized to avoid the peak of the photobleaching spectra supporting the result of spectroscopic measurements.
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