Influence of evaporative demand on aquaporin expression levels and hydraulic conductivity in the roots under natural weather condition

• Project/Area Number: 24570034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ecology/Environment
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: MURAI Mari 国立研究開発法人農業・食品産業技術総合研究機構, 東北農業研究センター生産環境研究領域, 上級研究員 (00343971)
• Co-Investigator(Kenkyū-buntansha): ISHIKAWA Junko 国立研究開発法人農業・食品産業技術総合研究機構, 次世代作物開発研究センター 稲研究領域, 主任研究員 (40343959)
• Co-Investigator(Renkei-kenkyūsha): KUWAGATA Tsuneo 国立研究開発法人農業・食品産業技術総合研究機構, 農業環境変動研究センター気候変動対応研究領域・作物温暖化応答, ユニット長 (90195602)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: アクアポリン / イネ / 水分生理 / 蒸散要求 / 天気

Outline of Final Research Achievements

Transpiration is major driving force for roots to uptake water from the soil. Aquaporins, the water channel proteins, are thought to play crucial roles in regulation of root hydraulic conductivity. In the present study, we showed that the evaporative demand plays a dominant role in the induction of specific aquaporins in rice roots in seedlings grown hydroponically and then transferred to an open field. We found that the abundance of the root-specific aquaporin transcripts, OsPIP2;4, OsPIP2;5 and OsTIP2;1 in the morning is highly correlated with the evaporative demand (potential evaporation). Aquaporin isoforms with higher correlation to potential evaporation showed higher diurnal amplitude of their expression. Based on these results, it is suggested that rice plants sense daily weather and respond to it by adjusting the expression of specific root aquaporin genes.

Research Products

• [Journal Article] Rice Plants Sense Daily Weather and Regulate Aquaporin Gene Expressions in the Roots -Close correlation with potential evaporation- (2015)
  • Author(s): Mari Murai-Hatano, Tsuneo Kuwagata, Hidehiro Hayashi, Junko Ishikawa-Sakurai, Masahisa Moriyama, Masumi Okada
  • Journal Title: Journal of Agricultural Meteorology Volume: 71 Pages: 1-12
  • NAID: 130005075872
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Rice aquaporin localizations in root, and the relationship between morphological changes and aquaporin gene expressions (2016)
  • Author(s): 林秀洋,村井麻理,石川淳子,冨永陽子,松波麻耶
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手県・盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] 天候による蒸散要求の変動がイネのアクアポリン発現の日周変化に及ぼす影響 (2014)
  • Author(s): 村井麻理,桑形恒男,石川淳子,林秀洋
  • Organizer: 第55回 日本植物生理学会年会
  • Place of Presentation: 富山大学 五福キャンパス
• [Presentation] イネはその日の気象条件を感知して根のアクアポリン発現量を調節している。 (2013)
  • Author(s): 村井麻理,桑形恒男,石川淳子,林秀洋
  • Organizer: 第58回低温生物工学会大会
  • Place of Presentation: 関西大学100周年記念会館
• [Presentation] Rice plants observe today’s weather and regulate aquaporin expressions in the roots. International Workshop on Plant Membrane Biology 2013 (2013)
  • Author(s): Murai-Hatano, M., Kuwagata, T., Ishikawa-Sakurai, J., Hayashi, H.
  • Organizer: International Workshop on Plant Membrane Biology 2013
  • Place of Presentation: 倉敷市芸文館
• [Presentation] Effect of low root-temperature on water use, growth and aquaporin expression in rice plants under various air-humidity conditions (2013)
  • Author(s): Tsuneo Kuwagata, Junko Ishikawa-Sakurai, Hidehiro Hayashi, Kiyoshi Nagasuga, Keiko Fukushi, Arifa Ahamed, Katsuko Takasugi, Maki Katsuhara
  • Organizer: International Workshop on Plant Membrane Biology 2013
  • Place of Presentation: 倉敷市芸文館