Does plant glutamate receptor function as a signaling molecules in stomatal closure?

Research Project

Project/Area Number	24570056
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Plant molecular biology/Plant physiology
Research Institution	Kagoshima University
Principal Investigator	YOSHIDA Riichiro 鹿児島大学, 農学部, 准教授 (70301786)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000) Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	シグナル伝達 / 気孔 / アミノ酸 / 植物ホルモン / 遺伝子発現 / シロイヌナズナ / 孔辺細胞 / グルタミン酸受容体 / 気孔応答
Outline of Final Research Achievements	Using physiological, genetic and molecular approaches, we have shown that amino acid glutamate (Glu) plays as a signaling molecule in both stomatal closure and the expression of pathogen resistant gene in higher plants. Glu dependent stomatal closure required Glu receptor gene GLR3.5 and calcium signaling. Glu induced the expression of PR-1 gene and this induction was mainly due to an increased level of endogenous salicylic acid (SA) and up-regulation of the SA biosynthetic gene expression. These results strongly supported the functional Glu signaling in higher plants and also provoked some evolutional commonalities in cellular signaling conserved between plants and mammals.