Studies of multiple functions of antimicrobial peptides and their physiological roles in the host innate immune system.
| Project/Area Number |
24570077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KIKUYAMA Sakae 早稲田大学, 教育・総合学術院, 名誉教授 (20063638)
KOBAYASHI Tetsuya 埼玉大学, 大学院理工学研究科, 教授 (00195794)
HASUNUMA Itaru 東邦大学, 理学部, 講師 (40434261)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 抗菌ペプチド / 生体防御ペプチド / 両生類 / ハーダー腺 / カセリシジン / ヒストン / DT40細胞 / cathelicidin / LAH-2細胞 / リュウキュウアカガエル / catesbeianalectin / ウシガエル / 抗酸化ペプチド |
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| Outline of Final Research Achievements |
We have cloned cDNAs encoding antimicrobial peptide (AMP) precursors from total RNA samples prepared from amphibian skin, Harderian gland, and brain and cells of the avian cell line DT40 using RT-PCR techniques. We detected chemotactic and degranulation against mastocytoma, bacterial surface substance-binding, and/or lectin-like activities as well as antimicrobial activities in the synthetic replicates of these AMPs such as bullfrog catesbeianalectin (CL) and chicken cathelicidin-B1 (chCATH-B1). We also detected the bacterial toxins-inducible chCATH-B1gene expression in DT40 cells. In addition, we showed directed antimicrobial activities and properties of Arg-rich histones (H3 and H4) and Lys-rich histone (H2B) against Gram-positive bacterium Staphylococcus aureus. While the Arg-rich histones disrupted the bacterial cell membrane by forming blebs, the Lys-rich histone inhibited the bacterial cell growth without morphological changes.
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Report
(4 results)
Research Products
(43 results)
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[Presentation] Molecular cloning, localization analysis and multifunctional characterization of host defense peptides in the tongue of Rana catesbeiana.2014
Author(s)
Shishido S, Omori S, Kondo H, Konishi Y, Sagae N, Hasunuma I, Iwamuro S, Kikuyama S, Kobayashi T.
Organizer
The 8th International Symposium on Amphibian and Reptalian Endocrinology and Neurobilogy
Place of Presentation
National Institute for Basic Biology, Okazaki, Japan
Year and Date
2014-11-07 – 2014-11-09
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