| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Photosynthetic organisms have a wide variety of storage glucans. To clarify the evolutionary processes that led to syntheses of starch and β-glucan, we performed the following analyses: 1. Activities and expression patterns of glucan synthesis-related enzymes were compared between the primitive rhodophytes Cyanidioschyzon (semi-amylopectin-type) and Cyanidium (glycogen-type). To examine roles of the enzymes isoamylase and α-glucan brancing enzyme, disruptants of the genes were constructed in Cyanidioschyzon and the structures of the glucan were characterized. 2. The nucleotide sequence of KRE6 homologue obtained from the haptophyte Pleurochrysis cDNA library has amino acid residues conserved in β-glucan binding proteins. Knockdown of this gene was examined to identify a function of the gene product.
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