Structural study of florigen and the receptor complex
Project/Area Number |
24570133
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
|
Research Institution | Kyoto University (2014) Nara Institute of Science and Technology (2012-2013) |
Principal Investigator |
OHKI Izuru 京都大学, 工学(系)研究科(研究院), 研究員 (80418574)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | X線結晶解析 / NMR / 植物 / 植物ホルモン / 構造生物学 / 花成 / フロリゲン / 核磁気共鳴 |
Outline of Final Research Achievements |
Florigen is a mobile plant hormone that determines the flowering time in higher plants, but its regulatory mechanism remains unclear. In this study, we focused on an anti-florigen, which suppresses flowering antagonizing with florigen, to clarify the role in floral regulation. From structural and biochemical analysis, anti-florigen was found to form a stable complex with florigen receptor and inhibit formation of florigen-receptor complex. The bindings of florigen and anti-florigen to receptor were exchangeable, suggesting that the balance of the two proteins is important for determining floral transition.
|
Report
(4 results)
Research Products
(15 results)
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[Journal Article] The crystal structure of the plant small GTPase OsRac1 reveals its mode of binding to NADPH oxidase2014
Author(s)
Kosami K, Ohki I, Nagano M, Furuita K, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
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Journal Title
J Biol Chem
Volume: 289
Pages: 28569-78
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac12014
Author(s)
Kosami K, Ohki I, Hayashi K, Tabata R, Usugi S, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
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Journal Title
Acta Crystallogr F Struct Biol Commun
Volume: 70
Pages: 113-5
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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