Analysis of ubiquitin receptors on regulatory functions of protein degradation

• Project/Area Number: 24570160
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Okayama University
• Principal Investigator: KOBAYASHI HIDEKI 岡山大学, 学内共同利用施設等, 教授 (20150394)
• Co-Investigator(Kenkyū-buntansha): SEKIGUCHI Takeshi 九州大学, 医学(系)研究科(研究院), 助教 (60187846) ; 山本 泰 岡山大学, 自然科学研究科, 教授 (40091251)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO Yasushi (40091251)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞内タンパク質分解 / ユビキチン / 塩ストレス / Gタンパク質 / 栄養ストレス / 葉緑体 / 光ストレス / タンパク質分解 / TORC1複合体 / チラコイド膜 / プロテアーゼ / UBL-UBAタンパク質 / Dsk2 / Irc22 / ユビキチンレセプター / 高塩ストレス / Gtr1-Gtr2

Outline of Final Research Achievements

Ubiquitin-dependent protein degradation plays important roles in stress responses of extra- and intra-cellular environment. In this study, we analyzed the regulation of protein degradation in stress responses using budding yeast and chloroplast. The results indicate that UBL-UBA protein Dsk2 is involved in salt stress, Gtr1-Gtr2 complex is involved in nutrient stress, and FtsH-thylakoid structure is involved in D1 degradation in chloroplast.

Research Products

• [Journal Article] Role of Auf1 in elimination of oxidatively damaged messenger RNA in human cells. (2015)
  • Author(s): Ishii T, Hayakawa H, Sekiguchi T, Adachi N, Sekiguchi M.
  • Journal Title: Free Radic Biol Med. Volume: 79 Pages: 109-116
  • DOI: 10.1016/j.freeradbiomed.2014.11.018
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Amino acid residues required for Gtr1p-Gtr2p complex formation and its interactions with the Ego1p-Ego3p complex and TORC1 components in yeast (2014)
  • Author(s): Takeshi Sekiguchi, Yoshiaki Kamada, Nobuaki Furuno, Minoru Funakoshi and Hideki Kobayashi
  • Journal Title: Genes to Cells Volume: 19 Issue: 6 Pages: 1-15
  • DOI: 10.1111/gtc.12145
  • Peer Reviewed / Open Access
• [Journal Article] Food habits of the urban Japanese weasels Mustela itatsi revealed by faecal DNA analysis. (2014)
  • Author(s): Okawara Y, Sekiguchi T, Ikeda A, Miura S, Sasaki H, Fujii T and Kaneko Y.
  • Journal Title: Mammal Study Volume: 39 Issue: 3 Pages: 155-161
  • DOI: 10.3106/041.039.0304
  • NAID: 40020212577
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Quality control of Photosystem II: Direct imaging of the changes in the thylakoid structure and ldistribution of FRtsH proteases in spinachchloroplasts under light stress. (2014)
  • Author(s): Yoshioka-Nishimura M, Nanba,D, Takaki, T, Ohba C, Tsumura N, Morita N, Sakamoto H, Murata K, Yamamoto Y.
  • Journal Title: Plant Cell Physiol. Volume: 55 Issue: 7 Pages: 1255-1265
  • DOI: 10.1093/pcp/pcu079
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Yeast Irc22 is a novel Dsk2-interacting protein that is involved in salt tolerance. (2014)
  • Author(s): Ishii T, Funakoshi M, Kobayashi H, Sekiguchi T.
  • Journal Title: Cells Volume: 3 Issue: 2 Pages: 180-198
  • DOI: 10.3390/cells3020180
  • Peer Reviewed
• [Journal Article] Quality Control of PSII: Behavior of PSII in the Highly Crowded Grana Thylakoids Under Excessive Light. (2014)
  • Author(s): Yamamoto Y, Kai S, Ohnishi A, Tsumura N, Ishikawa T, Hori H, Morita N, Ishikawa Y.
  • Journal Title: Plant and Cell Physiology Volume: in press Issue: 7 Pages: 1206-1215
  • DOI: 10.1093/pcp/pcu043
• [Journal Article] Quality control of Photosystem II: The molecular basis for the action of FtsH protease and the dynamics of the thylakoid membranes. (2014)
  • Author(s): Yoshioka-Nishimura M, Yamamoto Y
  • Journal Title: Journal of Photochemistry and Photobiology B: Biology Volume: in press Pages: 100-106
  • DOI: 10.1016/j.jphotobiol.2014.02.012
  • NAID: 120005434944
• [Journal Article] A New Subspecies Identification and Population Study of the Asian Small-clawed Otter (Aonyx cinereus) in the Malay Peninsula and Southern Thailand Based on Fecal DNA Method. (2014)
  • Author(s): Rosli M.K.A., Syed-Shabthar S.M.F., Abdul-Patah P., Abdul-Samad Z., Abdul S.N, Burhanuddin M.N., Zulkifli N.A., Shukor M.N., Budsabong K., Changtragoon S., Sekiguchi T, Sasaki H, Md-Zain B.M.
  • Journal Title: The Scientific World Journal Volume: 2014 Pages: 1-11
  • DOI: 10.1155/2014/457350
  • Peer Reviewed
• [Journal Article] Quality cotrol of photosystem II: Reversible and irreversible protein aggregation decides the fate of photosystem II under excessive illumination. (2013)
  • Author(s): Yamamoto Y, Hori H, Kai T, Ishikawa A, Ohnishi N, Tsumura N, and Morita N.
  • Journal Title: Frontiers in Plant Science Volume: 4
  • DOI: 10.3389/fpls.2013.00433
  • Peer Reviewed
• [Journal Article] Search for proteins required for accurate gene expression under oxidative stress: Roles of guanylate kinase and RNA polymerase (2013)
  • Author(s): Hchiro Inokuchi, Riyoko Ito, Tkeshi Sekiguchi, Mutsuo Skiguchi
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 46 Pages: 32952-32962
  • DOI: 10.1074/jbc.m113.507772
  • Peer Reviewed
• [Journal Article] Elimination and utilization of oxidized Guanine nucleotides in the synthesis of RNA and its precursors. (2013)
  • Author(s): Sekiguchi T, Ito R, Hayakawa H, Sekiguchi M.
  • Journal Title: J Biol Chem Volume: 288 Pages: 8128-8135
  • Peer Reviewed
• [Journal Article] Quality Control of Photosystem II: Lipid Peroxidation Accelerates Photoinhibition under Excessive Illumination. (2012)
  • Author(s): Tiffanie Chan
  • Journal Title: PLoS ONE Volume: 7(12) Issue: 12 Pages: e52100-e52100
  • DOI: 10.1371/journal.pone.0052100
  • Peer Reviewed
• [Presentation] ヘテロ２量体Gタンパク質のGtr1-Gtr2複合体におけるEgo1p-Ego3p複合体とTor複合体１との相互作用に必要なアミノ酸残基の同定 (2014)
  • Author(s): 関口猛、鎌田芳彰、古野伸明、舟越稔、小林英紀
  • Organizer: 第４回TOR研究会
  • Place of Presentation: 岡崎
  • Year and Date: 2014-09-17
• [Presentation] 大腸菌における８－オキソグアニンを含むヌクレオチドの排除機構 (2013)
  • Author(s): 伊東理世子, 井口八郎, 関口猛, 関口睦夫
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 横浜
• [Presentation] 酸素ストレス下の遺伝子発現機構 (2013)
  • Author(s): 井口八郎, 伊東理世子, 関口猛, 関口睦夫
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 横浜
• [Presentation] Elimination of oxidized nucleic acid (2013)
  • Author(s): Sekiguchi T, Itoh R, Hayakawa H, Sekiguchi M
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] HeLa細胞に導入したグアニンヌクレオチドの運命 (2012)
  • Author(s): 関口猛、早川浩、関口睦夫
  • Organizer: 遺伝学会第84回大会
  • Place of Presentation: 九州大学（福岡）
• [Presentation] Specific cleavage of oxidatively damaged RNA for accurate protein synthesis (2012)
  • Author(s): Takeshi Sekiguchi, Riyoko Ito, Hiroshi Hayakawa, and Mutsuo Sekiguchi
  • Organizer: Gordon Research Conferences
  • Place of Presentation: Newport (USA)
  • Invited