Why the energy transduction efficiency of F1-ATPase so high?

• Project/Area Number: 24570187
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Chuo University
• Principal Investigator: MUNEYUKI Eiro 中央大学, 理工学部, 教授 (80219865)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 生体エネルギー変換 / 分子モーター / F1-ATPase / 非平衡 / ATP / 自由エネルギー / 生体エネルギー論 / 変異体

Outline of Final Research Achievements

F1-ATPase is a highly efficient molecular motor. In order to understand the high efficiency, we compared the high efficient wild type motor with a low efficient mutant (beta G158A, T165S, Y341W), which is less sensitive to ADP inhibition and generates less torque. We found that this mutant motor dissipates "heat" in the rotational degree of freedom than wild type. We could achieve a quantitative measurement on the energy transduction by biological molecular motor which must be useful for understanding of energy transduction.

Research Products

• [Journal Article] Single molecule thermodynamics of ATP synthesis by F 1 -ATPase (2015)
  • Author(s): Shoichi Toyabe, Eiro Muneyuki
  • Journal Title: New Journal of Physics Volume: 17 Issue: 1 Pages: 1-7
  • DOI: 10.1088/1367-2630/17/1/015008
  • Peer Reviewed / Open Access
• [Journal Article] CHARMM Force-fields with Modified Polyphosphate Parameters Allow Stable Simulation of the ATP-Bound Structure of Ca2+-ATPase (2014)
  • Author(s): Yasuaki Komuro, Suyong Re, Chigusa Kobayashi, Eiro Muneyuki and Yuji Sugita
  • Journal Title: Journal of Chemical Theory and Computation Volume: 10 Issue: 9 Pages: 4133-4142
  • DOI: 10.1021/ct5004143
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Torque generation of Enterococcus hirae V-ATPase (2014)
  • Author(s): Ueno H, Minagawa Y, Hara M, Rahman S, Yamato I, Muneyuki E, Murata T, and Iino R
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Issue: 45 Pages: 31212-31223
  • DOI: 10.1074/jbc.m114.598177
  • Peer Reviewed / Open Access
• [Journal Article] Thermodynamic analyses of nucleotide binding to an isolated monomeric β subunit and the α3β3γ subcomplex of F1-ATPase. (2013)
  • Author(s): Kikuchi, Y., Naka, Y., Osakabe, H., Okamoto, T., Masaike, T., Ueno, H., Toyabe, S., Muneyuki, E.
  • Journal Title: Biophysical Journal Volume: 105 Issue: 11 Pages: 2541-2548
  • DOI: 10.1016/j.bpj.2013.10.018
  • Peer Reviewed
• [Journal Article] Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase (2013)
  • Author(s): Yoshihiro Minagawa, Hiroshi Ueno, Mayu Hara, Yoshiko Ishizuka-Katsura, Noboru Ohsawa,Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama, Ichiro Yamato, Eiro Muneyuki, Hiroyuki Noji, Takeshi Murata, and Ryota Iino
  • Journal Title: J.Biol.Chem Volume: 288 Issue: 45 Pages: 32700-32707
  • DOI: 10.1074/jbc.m113.506329
  • Peer Reviewed
• [Journal Article] Energetics of the presequence-binding poses in mitochondrial protein import through Tom20. (2013)
  • Author(s): Komuro Y, Miyashita N, Mori T, Muneyuki E, Saitoh T, Kohda D, Sugita Y.
  • Journal Title: J Phys Chem B Volume: 117 Issue: 10 Pages: 2864-71
  • DOI: 10.1021/jp400113e
  • Peer Reviewed
• [Journal Article] Properties of the electrogenic activity of bacteriorhodopsin. (2013)
  • Author(s): Miyazaki S, Matsumoto M, Brier SB, Higaki T, Yamada T, Okamoto T, Ueno H, Toyabe S, Muneyuki E.
  • Journal Title: Eur Biophys J. Volume: 42 Issue: 4 Pages: 257-65
  • DOI: 10.1007/s00249-012-0870-0
  • Peer Reviewed
• [Presentation] 外力存在下のF1-ATPaseの回転観察 (2015)
  • Author(s): 菊池洋輔，鳥谷部祥一，上野博史，宗行英朗
  • Organizer: 2015年生体運動研究合同班会議
  • Place of Presentation: 東京 学習院大学
  • Year and Date: 2015-01-07 – 2015-01-09
• [Presentation] 改良した多リン酸力場を用いた ATP/ADP 結合状態の筋小胞体カルシウムポンプの分子動力学計算 (2014)
  • Author(s): 小室 靖明, 李 秀栄, 小林 千草, 宗行 英朗, 杉田 有治
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-25 – 2015-09-27
• [Presentation] F1-ATPase のP-loop変異体TF1(βG158A)に対するリン酸の阻害効果 (2014)
  • Author(s): 星名 仁志, 吉田 光, 伊藤 亜由美, 伊藤 丈太郎, 鳥谷部 祥一, 上野 博史, 宗行 英朗
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Nucleotide binding to TF1 β subunit in relation to the effect of Pi (2014)
  • Author(s): 長埜 陸, 小原 清志, 上野 博史, 宗行 英朗
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Rotor-Stator Interactions in V1 and Vo from Enterococcus hirae V-ATPase (2014)
  • Author(s): 上野 博史, 皆川 慶嘉, 原 舞雪, 山登 一郎, 野地 博行, 村田 武士, 飯野 亮太, 宗行 英朗
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Observation of the rotation of F1-ATPase under the constant external torque (2014)
  • Author(s): 菊池 洋輔, 上野 博史, 中山 隆宏, 宗行 英朗, 鳥谷部 祥一
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] TF1 β;E190D変異体の外部トルク依存性 (2014)
  • Author(s): 田中 真奈, 河上 知広, 鳥谷部 祥一, 上野 博史, 工藤 正史, 宗行 英朗
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Single molecule study of a molecular motor: from biochemistry to physics (2013)
  • Author(s): Eiro Muneyuki
  • Organizer: Solvay Workshop on THERMODYNAMICS OF SMALL SYSTEMS
  • Place of Presentation: Université Libre de Bruxelles
  • Invited
• [Presentation] F1-ATPaseβサブユニットへのヌクレオチド結合の解析 (2013)
  • Author(s): 菊池 洋輔、宗行 英朗
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 広島大学
• [Presentation] The relationship between F1-ATPase P-loop mutants and Pi release (2013)
  • Author(s): Hikaru Yoshida, Ayumi Ito, Jotaro Ito, Tomoko Masaike, Takayuki Nishizaka, Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Observation of the rotation of F1-ATPase (2013)
  • Author(s): Yohsuke Kikuchi, Takahiro Nakayama, Shoichi Toyabe, Eiroh Muneyuki
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Comparison of the nucleotide binding to the isolated βsubunit and the F1-ATPase using the Sopped-Flow method (2013)
  • Author(s): Riku Nagano, Kiyoshi Obara, Tomoko Masaike, Hiroshi Ueno, Eiro Muneyuki
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Molecular dynamics simulations of SR Ca2+-ATPase using improved ATP force field (2013)
  • Author(s): Yasuaki Komuro, Chigusa Kobayashi, Suyong Re, Eiro Muneyuki, Yuji Sugita
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Conformational analysis of PA-glycans by replica-exchange molecular dynamics simulations (2013)
  • Author(s): Shigehisa Watabe, Suyong Re, Eiro Muneyuki, Yuji Sugita
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] F1－ATPase βサブユニットへのヌクレオチド結合の解析 (2013)
  • Author(s): 菊池 洋輔、＊宗行 英朗
  • Organizer: 2013年 生体運動研究合同班会議
  • Place of Presentation: 広島大学
• [Presentation] 分子モーター F1-ATPase によるエネルギー変換特性 (2012)
  • Author(s): 宗行英朗
  • Organizer: 第26回分子シミュレーション討論会
  • Place of Presentation: 九州大学 西新プラザ
  • Invited
• [Presentation] Single molecule energetics of F1-ATPase motor (2012)
  • Author(s): Shoichi Toyabe, TakahiroWatanabe-Nakayama, Hiroshi Ueno, Tetsuaki Okamoto, Hiroshi Taketani, Seishi Kudo, *Eiro Muneyuki
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
  • Invited
• [Presentation] Effect of nucleotide structure on nucleotide binding and rotation of F1-ATPase (2012)
  • Author(s): Yohsuke Kikuchi, Masaike Tomoko, Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] Inhibitory effect of Pi on F1-ATPase P-loop mutant (2012)
  • Author(s): Hikaru Yoshida, Ayumi Ito,Jotaro Ito, Syoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] Simultaneous observation of fluorescent-nucleotides and rotation of rotary molecular motor on plasmonic metal nanostructures (2012)
  • Author(s): Hiroshi Ueno, Shoichi Toyabe, Eiro Muneyuki
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] Molecular dynamics simulations of SRCa2+-pump in the ATP bound forms (2012)
  • Author(s): Yasuaki Komuro, Chigusa Kobayashi, Eiro Muneyuki, Yuji Sugita
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] Effect of external torque on the rotation of TF1 βE190D mutant (2012)
  • Author(s): Tomohiro Kawakami, Shoici Toyabe, Hiroshi Ueno, Seishi Kudo, Eiro Muneyuki
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Book] DOJIN BIOSCIENCE SERIES 17 一分子生物学 （原田 慶恵 石渡 信一 編 ） (2014)
  • Author(s): 上野博史, 鳥谷部祥一, 宗行英朗
  • Total Pages: 14
  • Publisher: 化学同人
• [Book] 化学フロンティアシリーズ 23 1分子ナノバイオ計測 （野地博行 編 ） (2014)
  • Author(s): 鳥谷部祥一，宗行英朗
  • Total Pages: 8
  • Publisher: 化学同人
• [Remarks] 最近の研究から
  • URL: http://www.phys.chuo-u.ac.jp/j/muneyuki/?%BA%C7%B6%E1%A4%CE%B8%A6%B5%E6%A4%AB%A4%E9