the mechanism of development regulated by the nucleocytoplasmic transport

• Project/Area Number: 24570215
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: 独立行政法人医薬基盤研究所 (2014); Osaka University (2012-2013)
• Principal Investigator: YASUHARA Noriko 独立行政法人医薬基盤研究所, その他部局等, 研究員 (90423152)
• Co-Investigator(Renkei-kenkyūsha): TAKAOKA Kastuyoshi 大阪大学, 大学院生命機能研究科, 助教 (90551044)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 核輸送 / 分化 / 発生 / ノックアウトマウス / 初期胚発生 / 核ー細胞質間輸送 / 核―細胞質間分子輸送 / importinα / 情報伝達制御

Outline of Final Research Achievements

To clarify the mechanism of development regulated by the nucleocytoplasmic transport, we attempted to create a knockout mouse targeting importin alpha, a typical nuclear transport receptor, and succeeded in obtaining the hetero knockout lines, of which the phenotype is now under analysis.

Research Products

• [Journal Article] Transcriptional program of Kpna2/Importin-α2 regulates cellular differentiation-coupled circadian clock development in mammalian cells. (2014)
  • Author(s): Yasuhiro Umemura, Nobuya Koike, Tsuguhiro Matsumoto, Seung-Hee Yoo, Zheng Chen, Noriko Yasuhara, Joseph S. Takahashi, Kazuhiro Yagita
  • Journal Title: PNAS Volume: 111 Pages: 5039-5048
  • Peer Reviewed / Open Access
• [Journal Article] Structural basis for the selective nuclear import of the C2H2 zinc-finger protein Snail by importin β. (2014)
  • Author(s): Choi Saehae. Yamashita Eiki, Yasuhara Noriko, Song Jinsue, Son Se‐Young, Won Young Han, Hong Hye Rim, Shin Yoon Sik, Sekimoto Toshihiro, Park Il Yeong, Yoneda Yoshihiro, Lee Soo Jae
  • Journal Title: Acta Crystallographica Section D Volume: 70 Pages: 1050-1060
  • Peer Reviewed
• [Journal Article] Importin alpha subtypes determine differential transcription factor localization in embryonic stem cells maintenance. (2013)
  • Author(s): Noriko Yasuhara, Ryosuke Yamagish, Yoshiyuki Arai, Rashid Mehmood, Chihiro Kimoto, Toshiharu Fujita, Kenichi Touma, Azumi Kaneko, Yasunao Kamikawa, Tetsuji Moriyama, Toshio Yanagida, Hiroki Kaneko, Yoshihiro Yoneda.
  • Journal Title: Developmental Cell Volume: 26 Pages: 123-135
  • Peer Reviewed
• [Journal Article] Improvement in protocol to generate homogeneous glutamatergic neurons from mouse embryonic stem cells reduced apoptosis. (2013)
  • Author(s): Ryosuke Fujiki, Akinobu Sato, Katsuhiko Hata, Fumi Tashiro, Noriko Yasuhara, Jun-ichi Miyazaki, Yoshihiro Yoneda, Masashi Fujitani, Toshihide Yamashita.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 432 Pages: 604-609
  • Peer Reviewed
• [Presentation] (3)核ー細胞質間分子輸送と細胞分化 (2014)
  • Author(s): 安原徳子
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良
  • Year and Date: 2014-06-11 – 2014-06-13
  • Invited
• [Presentation] Importinα regulates the stem cell differentiation (2012)
  • Author(s): 安原徳子、米田悦啓
  • Organizer: 第45回日本発生生物学会･第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸
• [Presentation] Recognition mode of basic NLS sequence by importin α1 (2012)
  • Author(s): 木本千裕、安原徳子、米田悦啓
  • Organizer: 第45回日本発生生物学会･第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸
• [Presentation] 核―細胞質間タンパク質輸送システムによる幹細胞のエピゲノム制御
  • Author(s): 安原徳子、山岸良介、木本千裕、金子寛生、米田悦啓
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] The novel role of the nucleocytoplasmic transport system in cell differentiation
  • Author(s): Noriko Yasuhara, Yoshihiro Yoneda
  • Organizer: 核ダイナミクス・染色体ワークショップ合同meeting
  • Place of Presentation: 淡路島