Study of non-coding RNA machinery complex-chromatoid body and dosage compensation.

• Project/Area Number: 24570237
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: University of East Asia (2014); Yamaguchi University (2012-2013)
• Principal Investigator: SATO YOKO 東亜大学, 医療学部, 教授 (50398963)
• Co-Investigator(Kenkyū-buntansha): OTOI Takeshige 山口大学, 共同獣医学部, 教授 (30311814)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 発生・分化 / 精子形成 / 遺伝子量補正 / 非コードRNA / クロマトイド小体 / 熱ストレス / 培養 / 精巣 / 発生、分化

Outline of Final Research Achievements

Nuage, a hazy electron dense structure, is thought to have the role for control of gene expression, exists unique to germ cells not to the somatic cells and localizes as chromatoid body in the post meiotic haploid round spermatid. During the spermiogenesis, the chromatoid body containing miRNA machinery, moves between the round spermatids connected by cytoplasmic bridges and thought to have the relation to the dosage compensation. In the present study, using germ cells and Sertoli cells co-culture system, we analyzed the timing of the dosage compensation after disturbing the movement of chromatoid body by physical and chemical method. Furthermore, we showed that the movement of chromatoid body is participate in the spermatogenesis under heat shock condition.

Research Products

• [Journal Article] Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C. (2014)
  • Author(s): Namula Z, Kodama R, Tanihara F, Morita Y, Sato Y, Wittayarat M, Taniguchi M, Otoi T.
  • Journal Title: Acta Vet Hung Volume: 62(1) Issue: 1 Pages: 106-116
  • DOI: 10.1556/avet.2013.046
  • Peer Reviewed
• [Journal Article] Cell cycle analysis and interspecies nuclear transfer of cat cells treated with chemical inhibitors. (2014)
  • Author(s): Wittayarat M, Fujiwara A, Chatdarong K, Techakumphu M, Sato Y, Tanihara F, Morita Y, Taniguchi M, Otoi T.
  • Journal Title: Acta Vet Hung Volume: 62(2) Issue: 2 Pages: 233-242
  • DOI: 10.1556/avet.2013.050
  • Peer Reviewed
• [Journal Article] Effect of sericin supplementation during in vitro maturation on the maturation, fertilization and development of porcine oocytes. (2014)
  • Author(s): Do LT, Namula Z, Luu VV, Sato Y, Taniguchi M, Isobe T, Kikuchi K, Otoi T.
  • Journal Title: Reprod Domest Anim. Volume: 49(2) Issue: 2
  • DOI: 10.1111/rda.12274
  • Peer Reviewed
• [Journal Article] Nuclear status and DNA fragmentation of oocytes from porcine, bovine and feline ovaries stored at 4 degrees C for 5 days. (2014)
  • Author(s): Luu VV, Namula Z, Do LT, Sato Y, Taniguchi M, Karja NW, Otoi T.
  • Journal Title: Cryo Letters. Volume: 35(1) Pages: 48-53
  • Peer Reviewed
• [Journal Article] Chelation of trace elements in preservation medium influences the quality of boar spermatozoa during liquid preservation at 5°C for 4 weeks. (2014)
  • Author(s): Taniguchi M, Wittayarat M, Morinaga K, Sato Y, Kin Do LT, Chatdarong K, Techakumphu M, Nii M, Otoi T.
  • Journal Title: Cryo Letters. Volume: 31(4) Pages: 336-344
  • Peer Reviewed
• [Journal Article] Establishment of adult mouse Sertoli cell lines by using the starvation method. (2013)
  • Author(s): Sato Y, Yoshida K, Nozawa S, Yoshiike M, Arai M, Otoi T, Iwamoto T.
  • Journal Title: Reproduction Volume: 145 (5) Issue: 5 Pages: 505-516
  • DOI: 10.1530/rep-12-0086
  • Peer Reviewed
• [Journal Article] Motility and fertility of boar semen after liquid preservation at 5°C for more than 2 weeks. (2013)
  • Author(s): Namula Z, Sato Y, Kodama R, Morinaga K, Luu VV, Taniguchi M, Nakai M, Tanihara F, Kikuchi K, Nagai T, Otoi T.
  • Journal Title: Anim Sci J. Volume: 84 (8) Issue: 8 Pages: 600-606
  • DOI: 10.1111/asj.12049
  • Peer Reviewed
• [Journal Article] The effect of relaxin supplementation of in vitro maturation medium on the development of cat oocytes obtained from ovaries stored at 4°C. (2013)
  • Author(s): Luu VV, Hanatate K, Tanihara F, Sato Y, Do LT, Taniguchi M, Otoi T.
  • Journal Title: Reprod Biol. Volume: 13 (2) Issue: 2 Pages: 122-126
  • DOI: 10.1016/j.repbio.2013.04.002
  • Peer Reviewed
• [Journal Article] Histone deacetylase inhibitor improves the development and acetylation levels of cat-cow interspecies cloned embryos. (2013)
  • Author(s): Wittayarat M, Sato Y, Do LT, Morita Y, Chatdarong K, Techakumphu M, Taniguchi M, Otoi T.
  • Journal Title: Cell Reprogram. Volume: 15 (4) Issue: 4 Pages: 301-308
  • DOI: 10.1089/cell.2012.0094
  • Peer Reviewed
• [Journal Article] Cryopreservation for bovine embryos in serum-free freezing medium containing silk protein sericin. (2013)
  • Author(s): Isobe T, Ikebata Y, Onitsuka T, Do LT, Sato Y, Taniguchi M, Otoi T.
  • Journal Title: Cryobiology. Volume: 67 (2) Issue: 2 Pages: 184-187
  • DOI: 10.1016/j.cryobiol.2013.06.010
  • Peer Reviewed
• [Journal Article] Comparison of activation ability between feline and bovine oocytes. (2013)
  • Author(s): Tanihara F, Kaedei Y, Namula Z, Luu VV, Sato Y, Wittayarat M, Taniguchi M, Otoi T.
  • Journal Title: Acta Vet Hung. Volume: 61 (4) Issue: 4 Pages: 491-494
  • DOI: 10.1556/avet.2013.032
  • Peer Reviewed
• [Journal Article] Effects of green tea polyphenol on the quality of canine semen after long-term storage at 5°C. (2013)
  • Author(s): Wittayarat M, Ito A, Kimura T, Namula Z, Luu VV, Do LT, Sato Y, Taniguchi M, Otoi T.
  • Journal Title: Reprod Biol. Volume: 13 (3) Issue: 3 Pages: 251-254
  • DOI: 10.1016/j.repbio.2013.07.006
  • Peer Reviewed
• [Journal Article] Cell cycle analysis and interspecies nuclear transfer of cat cells treated with chemical inhibitors. (2013)
  • Author(s): Wittayarat M, Fujiwara A, Chatdarong K, Techakumphu M, Sato Y, Tanihara F, Morita Y, Taniguchi M, Otoi T.
  • Journal Title: Acta Vet Hung. Volume: 11 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Studying spermatogenesis by using in vivo and in vitro models: Advantages and disadvantages of these models for practical use. (2012)
  • Author(s): Y.Sato, M. Taniguchi, T. Otoi
  • Journal Title: Veterinary Science and Technology Volume: 3 Issue: 01
  • DOI: 10.4172/2157-7579.1000115
  • Peer Reviewed
• [Journal Article] Glycoconjugates recognized by peanut agglutinin lectin in the inner acellular layer of the lamina propria of seminiferous tubules in human testes showing impaired spermatogenesis. (2012)
  • Author(s): Y. Sato, S. Nozawa, M. Yoshiike, T. Otoi, T. Iwamoto
  • Journal Title: Hum. Reprod Volume: 27 (6) Issue: 3 Pages: 659-668
  • DOI: 10.1093/humrep/der430
  • Peer Reviewed
• [Journal Article] Gene Suppression of Mouse Testis In Vivo Using Small Interfering RNA Derived from Plasmid Vectors (2012)
  • Author(s): Takizawa Ta, Ishikawa T, Kosuge T, Mizuguchi Y, Sato Y, Koji T, Araki Y, Takizawa To.
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 45 Issue: 1 Pages: 77-81
  • DOI: 10.1267/ahc.11024
  • NAID: 130001854310
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed
• [Journal Article] Estrogen-dependent regulation of sodium/hydrogen exchanger-3 (NHE3) expression via estrogen receptor β in proximal colon of pregnant mice (2012)
  • Author(s): Choijookhuu N, Sato Y, Nishino T, Endo D, Hishikawa Y, Koji T
  • Journal Title: Histochem.Cell Biol. Volume: 137 Issue: 5 Pages: 575-587
  • DOI: 10.1007/s00418-012-0935-2
  • NAID: 120006985370
  • Peer Reviewed
• [Journal Article] Follicle formation in the canine ovary after autografting to a peripheral site. (2012)
  • Author(s): T. Terazono, Y. Kaedei, F. Tanihara, Z. Namura, V. L. Viet, M. Takagi, M. Inoue, Y. Sato, M. Taniguchi and T. Otoi
  • Journal Title: Reprod. Domest. Anim. Volume: 47 (2) Issue: 2 Pages: 16-21
  • DOI: 10.1111/j.1439-0531.2011.01880.x
  • Peer Reviewed
• [Journal Article] Long-term preservation of chilled canine semen using vitamin C in combinationwith green tea polyphenol. (2012)
  • Author(s): M. Wittayarat, T. Kimura, R. Kodama, Z. Namula, K. Chatdarong, M. Techakumphu, Y. Sato, M. Taniguchi, T. Otoi
  • Journal Title: Cryo Letters Volume: 33(4) Pages: 318-326
  • Peer Reviewed
• [Journal Article] Effects of (-)-epigallocatechin gallate on the motility and penetrability of frozen-thawed boar spermatozoa incubated in the fertilization medium. (2012)
  • Author(s): Y. Kaedei, M. Naito, H. Naoi, Y. Sato, M. Taniguchi, F. Tanihara, K. Kikuchi, T. Nagai, T. Otoi
  • Journal Title: Reprod. Domest. Anim. Volume: 47(6) Issue: 6 Pages: 880-886
  • DOI: 10.1111/j.1439-0531.2012.01984.x
  • Peer Reviewed
• [Journal Article] Effect of sericin on preimplantation development of bovine embryos cultured individually. (2012)
  • Author(s): T. Isobe, Y. Ikebata, T. Onitsuka, M. Wittayarat, Y. Sato, M. Taniguchi and T. Otoi.
  • Journal Title: Theriogenology Volume: 78 (4) Issue: 4 Pages: 747-752
  • DOI: 10.1016/j.theriogenology.2012.03.021
  • Peer Reviewed
• [Presentation] Trichostatin a treatment effects on in vitro development of interspecies nuclear transfer cat embryos depend on recipient cytoplasm species. (2015)
  • Author(s): L.T.K.Do, Y.Sato, M.Taniguchi, T.Otoi
  • Organizer: 41th annual conference of international embryo transfer society
  • Place of Presentation: Versailles, France
  • Year and Date: 2015-01-10 – 2015-01-13
• [Presentation] Molecular analysis of testicular cells in Asian elephant (Elephas maximus) cryptorchid testis. (2014)
  • Author(s): Y. Sato, T. Tharasanit, N.Tipatanavattana, C. Somgird, C. Thitaram, S. Mahasawangkul, T. Otoi, M. Techakumphu
  • Organizer: The 12th International Symposimu on Spermatology 2014
  • Place of Presentation: Newcastle, Australia
  • Year and Date: 2014-08-10 – 2014-08-14
• [Presentation] Astaxanthin effects on maturation, fertilization and development of porcine oocytes cultured under heat stress. (2014)
  • Author(s): Do L.T., K., Luu V.V., Sato Y., Taniguchi M., Otoi T.
  • Organizer: 40th annual conference of International embryo transfer society
  • Place of Presentation: Reno, Nevada, US
• [Presentation] Ultrasonographic monitoring of canine ovaries clamped at subcutaneous site after hormone treatment. (2014)
  • Author(s): Terazono T., Luu V. V., Do L. T. K., Sato Y., Taniguchi M. Takagi M., Otoi T.
  • Organizer: 40th annual conference of international embryo transfer society
  • Place of Presentation: Reno, Nebada, US
• [Presentation] Long-term ovarian function after auto grafting to peripheral body site in a dog. (2013)
  • Author(s): Terazono T., Takagi M,, Akiyama K., Sato Y., Taniguchi M., Otoi T.
  • Organizer: The 3rd World Congress of the International Soceity for Fertility Preservation.
  • Place of Presentation: valencia, spain
• [Presentation] Effect of trichostatin A on in vitro embryo development of interspecies nuclear transfer embryos reconstructed from cat donor nuclei and bovine cytoplasm. (2013)
  • Author(s): M Wittayarat, Z. Namula, V.V. Luu, LTK Do, Y. Sato, M. Taniguchi, and T. Otoi
  • Organizer: 39th annual conference of International embryo transfer society
  • Place of Presentation: Hannover Congress Centrum, Hannover, Germany