| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Pleurotus pulmonarius is one of the major cultivated edible mushrooms in the world. We found a spontaneous mutant that shows the abnormal differentiation of both of pileus and stipe in the developmental stage of fruiting body. Despite the severe effect of occurrence of this mutation in the mushroom cultivation, the underlying cause is not entirely clear. In addition, many basic questions involving the biology of fruiting body formation remain unanswered. In this study, we narrowed down a candidate causes mutation to one gene by using the molecular techniques such as linkage analysis, comparative sequence analysis between wild type genome and its mutant, gene expression analysis and so on. This result may lead to the elucidation of the mechanism of this mutation. In future, we will verify the responsible gene for this mutation and develop the DNA markers for detection of this mutation.
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