| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
In order to screen for hypoallergenic wheat, seed storage proteins extracted from 265 lines of various hexaploid wheat were evaluated by Western blot using three kinds of anti-peptide antibodies for epitope of wheat allergy.
Gliadins were selectively extracted from Chinese Spring wheat and its aneuploid lines. Two-dimensional electrophoresis profiles were compared among lines. Out of 70 gliadin spots, 52 spots could be determined their chromosome loci. Furthermore, gliadins which have the epitope of wheat allergy and are transcribed from chromosome 6D were specifically suppressed in the tetrasomic 2A lines, suggesting Chinese Spring wheat has a specific gliadin suppressor on chromosome 2A.
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