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Taxonomic study of a Japanese native grape cultivar 'Koshu' through SNPs analysis and partial sequence of chloroplast DNA

Research Project

Project/Area Number 24580063
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionNational Research Institute of Brewing

Principal Investigator

Goto Nami  独立行政法人酒類総合研究所, 役員, 理事 (60372190)

Research Collaborator Sawler Jason  Dalhousie University
Myles Sean  Dalhousie University
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsブドウ / 甲州 / SNP / 葉緑体DNA / Vitis vinifera / 国際情報交換 / カナダ
Outline of Final Research Achievements

‘Koshu’ is a Japanese native grape cultivar and had been believed to be an oriental variety of Vitis vinifera; however, there were different opinions. Thus, to clarify the taxonomic position of ‘Koshu’, we carried out polymorphic DNA analyses. The result of single nucleotide polymorphisms (SNPs) analysis indicated that genomic DNA of ‘Koshu’ consists of slightly more than 70% of V. vinifera and slightly less than 30% of Asian wild species. In addition, the chloroplast DNA, which is inherited from the maternal ancestor, of ‘Koshu’ is a wild type and most closely related to that of a Chinese wild speciesV. davidii. From these results, it was revealed that ‘Koshu’ is a hybrid cultivar with V. davidii or closely related species as the maternal ancestor, even though the percentage of V. vinifera is higher than that of wild species.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) Remarks (3 results)

  • [Int'l Joint Research] Dalhousie大学(カナダ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Genetic analysis of East Asian grape cultivars suggests hybridization with wild Vitis2015

    • Author(s)
      Nami Goto-Yamamoto, Jason Sawler, Sean Myles
    • Journal Title

      PLoS ONE

      Volume: 10

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 日本各地で昔から栽培されているブドウのDNA解析2015

    • Author(s)
      後藤(山本)奈美
    • Organizer
      日本ブドウ・ワイン学会
    • Place of Presentation
      上越教育大学
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] DNA多型解析による甲州の分類に関する研究2014

    • Author(s)
      後藤奈美
    • Organizer
      日本醸造学会(技術賞・受賞講演)
    • Place of Presentation
      東京
    • Year and Date
      2014-10-07 – 2014-10-08
    • Related Report
      2014 Research-status Report
  • [Presentation] ‘甲州’のSNPs 解析及び葉緑体DNA 解析による分類学的検討

    • Author(s)
      後藤(山本)奈美1,河野弘美,沼田美子代,上用みどり,Jason SAWLER,Sean MYLES
    • Organizer
      日本ブドウ・ワイン学会
    • Place of Presentation
      山梨県甲府市
    • Related Report
      2013 Research-status Report
  • [Remarks] ’甲州’ブドウのルーツを解明

    • URL

      http://www.nrib.go.jp/data/nrtpdf/2013_1.pdf

    • Related Report
      2015 Annual Research Report
  • [Remarks] 甲州ブドウのルーツ

    • URL

      http://www.nrib.go.jp/sake/pdf/NRIBNo27.pdf

    • Related Report
      2014 Research-status Report
  • [Remarks] ‘甲州’ブドウのルーツを解明

    • URL

      http://www.nrib.go.jp/data/nrtpdf/2013_1.pdf

    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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