| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
One genomic region, which is induced by genistein, was found in Bradyrhizobium japonicum and named BjG30. The induction level reached maximum at 15 min after the addition of genistein and the induction profile was different from that of symbiosis genes (nod genes). The BjG30 composed of multidrug efflux pump, polyhydroxybutyrate metabolism, and transcriptional regulatory genes, and played an important role in symbiosis with soybean for the nodule formation and nitrogen fixation. In addition, the results of global gene expression analyses showed that the efflux of genistein would functionally regulate the expression levels of symbiosis genes.
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