| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Polyhydroxyalkanoates (PHAs), the biodegradable plastics produced by many microorganisms, have attracted interest as the substitute for petroleum-derived plastics. In this study, genetic engineering and global transcription machinery engineering (gTME) approach were employed for the representative methylotrophic bacterium with the aim of constructing microbial strain capable of efficient biosynthesis of practical PHA copolymers from methanol. Recombinant strain which accumulated PHA copolymer with higher second monomer composition was constructed by expression of gene encoding enzyme involved in providing second monomer. A random mutant library of rpoD gene encoding sigma 70 factor was developed for gTME and enabled the screening of strains improved PHA contents on high concentration of methanol.
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