| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
We attempted to elucidate the mechanism for hyperproduction of citric acid by Aspergillus kawachii. A. kawachii ligD-disruptant which exhibits the ability of homologous recombination with high efficiency was constructed and auxotrophic strains were constructed. Approximately 1,100 genes that show differential expression after shifting cultivation at high temperature to cultivation at low temperature were identified. Gene ontology analysis revealed that A. kawachii is stressed from cultivation at high temperature that enhances the amylase activity and responds to protect against heat damage. Cultivation of A. kawachii at low temperature enhances production of citric acid. Under this condition EMP pathway to form pyruvate was reinforced by the repression of metabolic pathways for synthesis of glycerol and trehalose, and pentose phosphate in A. kawachii. Thirty six genes were selected to be important for high production of citric acid and 35 genes were disrupted in A. kawachii.
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