| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Outline of Final Research Achievements |
A variety of oncogene and signal transduction proteins are known to be myristoylated. Although the role of myristoylation in protein-lipid interaction is well established, the involvement of myristoylation in protein-protein interaction is less well understood.
To elucidate the myristoylation-dependent protein-protein interaction, we detected the binding proteins of NAP22 in a miyristoyaltion-dependent manner. The binding proteins included the nuclear proteins such as HMGB1. Protein-protein interactions of HMGB1 and myristoylated NAP-22 were observed in cytosol and nuclear regions of various mammalian cells. These results suggest that myristoylation-dependent protein-protein interaction might be very significant to cellular function.
|
