Application and genetical investigation of novel cellulase produced by Hirondellea gigas
| Project/Area Number |
24580153
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
Kobayashi Hideki 国立研究開発法人海洋研究開発機構, 海洋生命理工学研究開発センター, 主任研究員 (40399564)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | セルラーゼ / ヨコエビ / 深海 / RNA / RNAseq / 遺伝子資源 / 超深海 / 無脊椎動物 / 消化酵素 / RNAシーケンス / 多糖分解酵素 / マリアナ海溝 / 小笠原海溝 / 超深海性ヨコエビ / 深海生物 |
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| Outline of Final Research Achievements |
Hirondellea gigas in the Challenger Deep has novel cellulase, however, its application is difficult because of Convention on Biological Diversity. Therefore, I researched amphipods in the Izu-Ogasawara Trench, and found that they have the cellulase same properties as H. gigas. I extracted total RNA from the amphipod from the Izu-Ogasawara Trench, and obtained RNA sequence data using Next Generation Sequencer. I found seven sequences related with cellulase in the RNA sequence data. I tried PCR amplification of cellulase genes from cDNA, however, I cannot obtain the same sequence as seven cellulase candidates. Therefore, there would be some mistakes in the assembling of sequence data.
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Report
(5 results)
Research Products
(11 results)
