Screening for anti-aging food that regulates intestinal function and clarification of its molecular basis
| Project/Area Number |
24580190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Kyushu University |
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Principal Investigator |
Yoshinori Katakura 九州大学, (連合)農学研究科(研究院), 准教授 (50264106)
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| Research Collaborator |
FUJII Kaoru
HARADA Gakuro
YAMASHITA Shuntaro
KADOOKA Keishi
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | アンチエイジング / 乳酸菌 / SIRT1 / がん抑制 / サーチュイン / 食品 / 老化 |
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| Outline of Final Research Achievements |
We used human colon cancer cell line, Caco-2 cells, and established recombinant Caco-2 cells expressing EGFP under the control of human SIRT1 promoter. We monitored the changes in EGFP fluorescence after adding lactic acid bacteria by using flow cytometer, and identified T2102 strain as positive lactic acid bacteria. T2102 activated SIRT1, deacetylated and destabilized β-catenin. T2102 repressed hTERT expression and induced cellular senescence in Caco-2 cells. Furthermore, tumor suppressing activity of T2102 was evidenced by anchorage-independent growth and tumorigenicity.
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Report
(5 results)
Research Products
(59 results)
