| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Outline of Final Research Achievements |
This study evaluated the genetic impact of the release of captive Sebastes oblongus and developed the cryopreservation method of spermatozoa to conserve the genetic diversity of the native population. The genetic diversity was determined based on nucleotide sequence data from the mtDNA control region and msDNA analysis of five year classes (before 2005 to 2009) of S. oblongus. Although the estimated pairwise Fst did not show the significant difference among year classes, the estimated haplotype diversity was recognized 7.4% decrease between before2005 and 2009 year class. We examined the method for cryopreserving spermatozoa judging from the mortility of thawed sperm. The best results were obtained when the spermatozoa were cryopreserved with diluents comprising 10% DMSO and fetal bovine serum (FBS). For freezing sperm samples, straws were placed at 10.0 cm above the surface of liquid nitrogen (LN) and plunged in LN. The straws were thawed in water bath at 20°C for 7 sec.
|
