| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Outline of Final Research Achievements |
Function and redistribution of intermediate filaments in the mammalian oocytes during maturation were examined by confocal laser scanning microscopy. In porcine oocytes, distributional changes of keratin and vimentin were not clear, but those of neurofilaments (NF), GFAP and nestin were clarified during oocyte maturation. Interestingly, it is suggested that NF, GFAP and nestin may be included in the movement of lipid droplets in the ooplasm and that NF may function in chromosomal segregation at the second meiotic division. Furthermore, desmin may participate in anchoring the meiotic apparatus to the cortical region of oocytes, collaborating with actin microfilaments. Effects of the cell-cycle regulating factors and Rho kinase on the cytoskeletal architecture of the intermediate filaments were also discussed.
|
