Role of Mitf, a tissue-specific transcription factor, in osteoclastogenesis and osteoclast function, and molecular mechanisms underlying Mitf-mediated osteoclastogenesis

• Project/Area Number: 24580437
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Azabu University
• Principal Investigator: MURAKAMI Masaru 麻布大学, 獣医学部, 教授 (80271360)
• Co-Investigator(Renkei-kenkyūsha): FUNABA Masayuki 京都大学, 大学院農学研究科, 准教授 (40238655)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 破骨細胞分化 / 転写因子 / Mitf / 転写因子Mitf

Outline of Final Research Achievements

Treatment with RANKL induces osteoclastogenesis in murine and canine bone marrow cells as well as murine RAW264.7 cells. Analyses of Mitf isoform expression indicated that Mitf-E gene was induced within 24 hours after RANKL treatment in all examined cells. Knockdown of Mitf-E by its siRNA transfection inhibited RANKL-induced osteoclastogenesis. In mouse cells, co-treatment with TGF-β enhanced RANKL-induced osteoclastogenesis, whereas TGF-β inhibited canine osteoclastogenesis induced by RANKL.

Research Products

• [Journal Article] Enhancement of RANKL-induced MITF-E expression and osteoclastogenesis by TGF-β (2014)
  • Author(s): Kumiko Asai, Masayuki Funaba and Masaru Murakami
  • Journal Title: Cell Biochem Funct Volume: in press Issue: 5 Pages: 401-409
  • DOI: 10.1002/cbf.3028
  • NAID: 120005623241
  • Peer Reviewed
• [Journal Article] Expression of activin receptor-like kinase 7 in adipose tissues. (2013)
  • Author(s): Murakami M. et al.
  • Journal Title: Biochem. Genet. Volume: 51 Issue: 3-4 Pages: 202-210
  • DOI: 10.1007/s10528-012-9555-8
  • Peer Reviewed
• [Presentation] BMPによるSmad8発現調節メカニズムの解析 (2014)
  • Author(s): 片川 優子、舟場 正幸、村上 賢
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-10
• [Presentation] TGF-βによるRANKL 誘導性Mitf-E の発現と破骨細胞分化への影響
  • Author(s): 浅井 久美子，舟場 正幸，村上 賢
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Regulatory expression of Smad8 by BMP
  • Author(s): Yuko Katakawa, Masahide Horiguchi, Masayuki Funaba, Masaru Murakami
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド