Analysis of suppression of Th2-type immune responses through innate immunity by glyco-polymer bearing plant antigenic N-glycans
| Project/Area Number |
24580494
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Okayama University |
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Principal Investigator |
MAEDA Megumi 岡山大学, 環境生命科学研究科, 助教 (20434988)
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Yoshinobu 岡山大学, 大学院環境生命科学研究科, 教授 (70195384)
OTSUKI Takemi 川崎医科大学, 衛生学, 教授 (40160551)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | N-グリカン / 植物抗原性糖鎖 / スギ花粉アレルゲン / Th2免疫応答抑制 / 多量精製 / ルイスa抗原 / 糖鎖ポリマー / 樹状細胞 / Cry j1 / Asn-糖鎖 / γポリグルタミン酸 / サイトカイン / 淡水性植物 / T細胞 / Th2応答 / 植物抗原性 / 親水性クロマト / γ-ポリグルタミン酸 / ヒト樹状細胞 |
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| Outline of Final Research Achievements |
The plant antigenic N-glycans from plant glycoproteins have suppressed Th2-type immune responses by Japanese cedar pollen allergen, suggesting that the plant antigenic N-glycans could become effective agents in therapy for patients with pollinosis. In this study, a new method with hydrophilic partitioning was developed to prepare the large-scale of Asn-glycopeptide bearing plant antigenic N-glycans from Ginkgo seeds. And then, plant antigenic N-glycans bearing Lewis a antigen(s) at the non-reducing terminal were found in submerged plants. Finally, the glycol-polymer bearing multivalent plant antigenic N-glycans was synthesized, and the differential inhibition of dendritic cells by the glycol-polymer was analyzed.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Purification and Substrate Specificity of A <i>Ginkgo biloba</i> Glycosidase Active in β-1,2-Xylosidic Linkage in Plant Complex Type <i>N</i>-Glycans2013
Author(s)
Maeda, M., Akiyama, T., Yokouchi, D., Woo, K. K. and Kimura, Y.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 77
Issue: 9
Pages: 1973-1976
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Journal Article] Large-Scale Preparation of Asn-Glycopeptide Carrying Structurally Homologous Antigenic <i>N</i>-Glycan2013
Author(s)
Maeda, M., Takeda, N., Mano, A., Yamanishi, M., Kimura, M., and Kimura, Y.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 77
Issue: 6
Pages: 1269-1274
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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