Study of the molecular mechanisms of brain sex differentiation of the SF-1 KO mouse

• Project/Area Number: 24590257
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Aichi Gakuin University
• Principal Investigator: IKEDA YAYOI 愛知学院大学, 歯学部, 教授 (00202903)
• Co-Investigator(Kenkyū-buntansha): IKEDA Masaaki 東京医科歯科大学, 医歯学総合研究科, 准教授 (20193211)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 脳の性分化 / 性ホルモン / ノックアウトマウス / エストロゲン / 脳の性差

Outline of Final Research Achievements

To study the molecular mechanisms of brain sex differentiation, we analyzed the expression of several markers in the SF-1 knockout (KO) mice using immunohistochemistry. The localization and the positive cell number for these markers of SF-1 KO male and female mice were similar to those of WT females, indicating that the two brain regions of SF-1 KO male mice are feminized. The results support the idea that perinatal gonadal hormones are required for masculinization of the brain.

Research Products

• [Journal Article] Newborn mice exposed prenatally to bisphenol A show hyperactivity and defective neocortical development (2014)
  • Author(s): Komada M, Itoh S, Kawachi K, Kagawa N, Ikeda Y, Nagao N
  • Journal Title: Toxicology Volume: 323 Pages: 51-60
  • DOI: 10.1016/j.tox.2014.06.009
  • Peer Reviewed
• [Journal Article] NR5A1 is required for functional maturation of Sertoli cells during postnatal development (2012)
  • Author(s): Kato T, Esaki M, Matsuzawa A, Ikeda Y
  • Journal Title: Reproduction Volume: (印刷中) Issue: 5 Pages: 663-672
  • DOI: 10.1530/rep-11-0365
  • Peer Reviewed
• [Presentation] A/Jマウス口蓋における骨形成因子の発現 (2015)
  • Author(s): 森 明弘, 井村英人, 池田やよい, 夏目長門
  • Organizer: 第39回日本口蓋裂学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-21 – 2015-05-22
• [Presentation] Newborn mice exposed prenatally to bisphenol A show hyperactivity and defective neocortical development (2015)
  • Author(s): Komada M, Itoh S, Kawachi K, Nagao T, Ikeda Y
  • Organizer: 第120回日本解剖学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] A rare case of inferior vena cava duplication (2015)
  • Author(s): Ito T, Hayakawa A, Takeuchi R, Ikeda Y
  • Organizer: 第120回日本解剖学会総
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Expression of Osterix in the cleft palate of A/J mouse embryo (2015)
  • Author(s): Mori A, Takahashi M, Komada M, Ikeda Y
  • Organizer: 第120回日本解剖学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 胎児期低用量ビスフェノールA曝露は発生・発達期の大脳皮質に形態・機能的異常を誘発する (2014)
  • Author(s): 駒田致和，長尾哲二，池田やよい，菅野純
  • Organizer: 第17回環境ホルモン学会研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2014-12-09
• [Presentation] Immunohistochemical detection of cyclin E in postmitotic neurons of the mouse adult hippocampal dentate gyrus (2014)
  • Author(s): Ikeda Y, Ikeda. MA
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington DC
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] 胎児期低用量ビスフェノールA暴露による大脳皮質における影響の神経発生毒性学的評価 (2014)
  • Author(s): 駒田致和、浅井泰子、守井見奈、松木美知枝、河内宏太、池田やよい、長尾哲二
  • Organizer: 第41回 日本毒性学会学術年会
  • Place of Presentation: 神戸
  • Year and Date: 2014-07-02 – 2014-07-04
• [Presentation] 大脳皮質形成におけるステロイドホルモンの局在と役割 (2014)
  • Author(s): 駒田致和、池田やよい
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 宇都宮
• [Presentation] SF-1ノックアウトマウスのPOAにおけるプロゲステロン受容体とkisspeptinの発現 (2013)
  • Author(s): 池田やよい、加藤朋子、加藤大貴、高橋幸子、駒田致和
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] 大脳皮質形成におけるステロイドホルモンの局在と役割 (2013)
  • Author(s): 駒田致和、池田やよい
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山
• [Presentation] Immunohistochemical localization of estrogen receptors alpha and beta, progesterone receptor, and kisspeptin in the preoptic area of SF-1 knockout mice (2013)
  • Author(s): Yayoi Ikeda, Tomoko Kato, Munekazu Komada
  • Organizer: Neuroscience 2013
  • Place of Presentation: San Diego
• [Presentation] 生後SF-1ノックアウトマウスPOAにおける脳の性分化マーカーの発現 (2013)
  • Author(s): 池田やよい, 加藤朋子, 駒田致和, 加藤大貴, 高橋幸子
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 香川
• [Presentation] SF-1ノックアウトマウスのPOAにおけるERaとPR発現の免疫組織学的解析 (2012)
  • Author(s): 池田やよい, 加藤朋子
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 生殖腺特異的SF-1ノックアウトマウスの性腺分化異常 (2012)
  • Author(s): 池田やよい、加藤朋子
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Invited