Analysis of mitochondria D-loop DNA/RNA

• Project/Area Number: 24590459
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses
• Principal Investigator: TOMITA Yasuhiko 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), その他部局等, その他 (60263266)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ミトコンドリア / ＶＣＰ / ＵＢＬ４Ａ / 発癌 / 細胞内局在 / VCP / ubuquitin like 4A / D-loop / 7SRNA / UBL4A / STAT3

Outline of Final Research Achievements

To clarify the distribution pattern of mitochondrial DNA and RNA in cultured cells, in situ hybridization and polymerase chain reaction was performed. However no distinct observation was obtained.
Ubiquitin like 4A (UBL4A) is a cofactor of valosin containing protein (VCP), which is associated with cancer metastasis. Inhibitory effect of UBL4A in STAT signaling was found. Chronic inflammation with steatosis of the pancreas appears to be an important predisposing factor for pancreatic cancer.

Research Products

• [Journal Article] Usefulness of dual phase 18F-FDG-PET/CT for diagnosing small pancreatic tumors. (2015)
  • Author(s): Kawada N, Uehara H, Hosoki T, Takami M, Shiroeda H, Arisawa T, Tomita Y
  • Journal Title: Pancreas Volume: 44 Issue: 4 Pages: 655-659
  • DOI: 10.1097/mpa.0000000000000313
  • Peer Reviewed
• [Journal Article] GdX/UBL4A Specifically Stabilizes the TC45/STAT3 Association and Promotes Dephosphorylation of STAT3 to Repress Tumorigenesis. (2014)
  • Author(s): Wang Y, Ning H, Ren F, Zhang Y, Rong Y, Wang Y, Su F, Cai C, Jin Z, Li Z, Gong X, Zhai Y, Wang D, Jia B, Qiu Y, Tomita Y, Sung JJ, Yu J, Irwin DM, Yang X, Fu X, Chin YE, Chang Z.
  • Journal Title: Mol Cell Volume: 53 Issue: 5 Pages: 752-765
  • DOI: 10.1016/j.molcel.2014.01.020
  • Peer Reviewed
• [Journal Article] Pancreatic Steatosis and Fibrosis as Predisposing Factors of Cancer Development (2014)
  • Author(s): Tomita Y, Azuma K, Nonaka Y, Kamada Y, Tomoeda M, Kishida M, Tanemura M, Miyoshi E
  • Journal Title: Pancreas Volume: 43 Issue: 7 Pages: 1032-1041
  • DOI: 10.1097/mpa.0000000000000159
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Insulin Receptor Substrate 1/2 (IRS1/2) Regulates Wnt/β-catenin Signaling through Blocking Autophagic Degradation of Dishevelled2. (2014)
  • Author(s): Geng Y, Ju Y, Ren F, Qiu Y, Tomita Y, Tomoeda M, Kishida M, Wang Y, Jin L, Su F, Wei C, Jia B, Li Y, Chang Z.
  • Journal Title: J Biol Chem. Volume: 18 Issue: 16 Pages: 11230-41
  • DOI: 10.1074/jbc.m113.544999
  • Peer Reviewed
• [Journal Article] Potential use of point shear wave elastography for the pancreas: A single center prospective study. (2014)
  • Author(s): Kawada N, Tanaka S, Uehara H, Ohkawa K, Yamai T, Takada R, Shiroeda H, Arisawa T, Tomita Y.
  • Journal Title: Eur J Radiol. Volume: 83 Issue: 4 Pages: 620-24
  • DOI: 10.1016/j.ejrad.2013.11.029
  • Peer Reviewed
• [Journal Article] Bilateral ovarian metastasis of non-small cell lung cancer with ALK rearrangement. (2014)
  • Author(s): Fujiwara A, Higashiyama M, Kanou T, Tokunaga T, Okami J, Kodama K, Nishino K, Tomita Y, Okamoto I.
  • Journal Title: Lung Cancer Volume: 83 Issue: 2 Pages: 302-4
  • DOI: 10.1016/j.lungcan.2013.11.022
  • Peer Reviewed
• [Journal Article] Cells of origin of squamous epithelium, dysplasia and cancer in the head and neck region after bone marrow transplantation. (2014)
  • Author(s): Kano Y, Ishii H, Konno M, Yamasaki M, Miyata H, Nishikawa S, Hamabe A, Ogawa H, Takahashi H, Ohta K, Hasegawa S, Tanaka K, Fukusumi T, Otsuka M, Kawamoto K, Haraguchi N, Fujimoto R, Isobe M, Tomita Y, Matsuura N, Takiguchi S, Mori M, Doki Y.
  • Journal Title: Int J Oncol Volume: 44 Issue: 2 Pages: 443-50
  • DOI: 10.3892/ijo.2013.2206
  • Peer Reviewed
• [Journal Article] miR-92a inhibits peritoneal dissemination of ovarian cancer cells by inhibiting integrin α5 expression (2013)
  • Author(s): Ohyagi-Hara C, Sawada K, Mabuchi S, Takahashi T, Kurachi H
  • Journal Title: Am J Pathol Volume: 182(5) Issue: 5 Pages: 1876-1889
  • DOI: 10.1016/j.ajpath.2013.01.039
  • Peer Reviewed
• [Journal Article] Intraoperative diagnosis using sentinel node biopsy with indocyanine green dye in gastric cancer surgery: an institutional trial by experienced surgeons. (2013)
  • Author(s): Miyashiro I, Hiratsuka M, Kishi K, Takachi K, Yano M, Takenaka A, Tomita Y, Ishiguro S.
  • Journal Title: Ann Surg Oncol Volume: 20 Issue: 2 Pages: 542-546
  • DOI: 10.1245/s10434-012-2608-8
  • Peer Reviewed
• [Journal Article] Histological features responsible for brownish epithelium in squamous neoplasia of the esophagus by narrow band imaging. (2013)
  • Author(s): Kanzaki H, Ishihara R, Ishiguro S, Nagai K, Matsui F, Yamashina T, Ohta T, Yamamoto S, Hanaoka N, Hanafusa M, Takeuchi Y, Higashino K, Uedo N, Iishi H, Tomita Y.
  • Journal Title: J Gastroenterol Hepatol Volume: 28 Issue: 2 Pages: 274-278
  • DOI: 10.1111/jgh.12059
  • Peer Reviewed
• [Journal Article] Role of ¹⁸F-fluorodeoxyglucose positron emission tomography/computed tomography in predicting the pathologic response to preoperative chemoradiation therapy in patients with resectable T3 pancreatic cancer. (2013)
  • Author(s): Kittaka H, Takahashi H, Ohigashi H, Gotoh K, Yamada T, Tomita Y, Hasegawa Y, Yano M, Ishikawa O.
  • Journal Title: World J Surg. Volume: 37 Issue: 1 Pages: 169-178
  • DOI: 10.1007/s00268-012-1775-x
  • Peer Reviewed
• [Journal Article] Surgery with vascular reconstruction for soft-tissue sarcomas in the inguinal region: oncologic and functional outcomes. (2012)
  • Author(s): Emori M, Hamada K, Omori S, Joyama S, Tomita Y, Hashimoto N, Takami H, Naka N, Yoshikawa H, Araki N.
  • Journal Title: Ann Vasc Surg. Volume: 26 Issue: 5 Pages: 693-699
  • DOI: 10.1016/j.avsg.2011.12.003
  • Peer Reviewed