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Immunosuppressions by extracellular vesicles secreted from Schistosoma japonicum

Research Project

Project/Area Number 24590503
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KUMAGAI Takashi  東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (40369054)

Co-Investigator(Renkei-kenkyūsha) OHTA Nobuo  東京医科歯科大学, 大学院医歯学総合研究科, 教授 (10143611)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords細胞外小胞 / 住血吸虫 / miRNA / 赤血球 / エクソソーム / マイクロベシクル / 免疫抑制 / 性成熟 / 分泌型小胞 / スフィンゴミエリナーゼ / GW4869 / BAPTA-AM / エキソソーム / IFN-g / IL-4 / IL-10
Outline of Final Research Achievements

Schistosoma japonicum, which is endemic in Asia, parasitizes in the blood vesicles of mammalian hosts. They takes erythrocytes for their reproductive activities. In present study, we focused the extracellular vesicles, which is widely observed in the organisms as vital mobile particles. As the results, the extra cellular vesicles secrete from adult worms, after male and female worms became to be paired, and after they took in the erythrocytes. Furthermore, we found abundant miRNAs, which regulate the targeting genes at the mRNA levels, in their vesicles. This means that the extracellular vesicles have roles on the male-female communications using gene-regulations by miRNAs. Finally, this vesicles also have roles on the suppression to Th1 cytokine productions. Taken together with our results, the extracellular vesicles secreted from S. japonicum, may be required in reproductive activities and immune evasions.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (23 results)

All 2015 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (14 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase (SjGST) DNA vaccine against murine Schistosoma japonicum infection.2015

    • Author(s)
      Mbanefo EC, Kumagai T, Kodama Y, Kurosaki T, Furushima-Shimogawara R, Cherif MS, Mizukami S, Kikuchi M, Huy NT, Ohta N, Sasaki H, Hirayama K.
    • Journal Title

      Parasitol Int.

      Volume: 64 Issue: 4 Pages: 24-31

    • DOI

      10.1016/j.parint.2015.01.005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A new surveillance and response tool: risk map of infected Oncomelania hupensis detected by Loop-mediated isothermal amplification (LAMP) from pooled samples.2015

    • Author(s)
      Tong QB, Chen R, Zhang Y, Yang GJ, Kumagai T, Furushima-Shimogawara R, Lou D, Yang K, Wen LY, Lu SH, Ohta N, Zhou XN.
    • Journal Title

      Acta Trop.

      Volume: 141 Pages: 170-177

    • DOI

      10.1016/j.actatropica.2014.01.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular genotyping of anisakis larvae in Middle Eastern Japan and endoscopic evidence for preferential penetration of normal over atrophic mucosa.2014

    • Author(s)
      Arai T, Akao N, Seki T, Kumagai T, Ishikawa H, Ohta N, Hirata N, Nakaji S, Yamauchi K, Hirai M, Shiratori T, Kobayashi M, Fujii H, Ishii E, Naito M, Saitoh S, Yamaguchi T, Shibata N, Shimo M, Tokiwa T.
    • Journal Title

      PLoS One.

      Volume: 9(2) Issue: 2 Pages: e89188-e89188

    • DOI

      10.1371/journal.pone.0089188

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Basophil depletion downregulates Schistosoma mansoni egg-induced granuloma formation2013

    • Author(s)
      Anyan, W.K., Seki, T., Kumagai, T., Obata-Ninomiya, K., Furushima-Shimogawara, R., Kwansa-Bentum, B., Akao, N., Bosompem, K.M., Boakye, D.A., Wilson, M.D., Karasuyama, H., and Ohta, N.
    • Journal Title

      Parasitol. Int.

      Volume: 62 Issue: 6 Pages: 508-513

    • DOI

      10.1016/j.parint.2013.07.003

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Contamination, distribution and pathogenicity of Toxocara canis and T. cati eggs from sandpits in Tokyo, Japan.2013

    • Author(s)
      Macuhova K, Akao N, Fujinami Y, Kumagai T, Ohta N.
    • Journal Title

      J Helminthol.

      Volume: 87(3) Issue: 3 Pages: 271-6

    • DOI

      10.1017/s0022149x12000314

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phylogenetic relationships of rat lungworm, Angiostrongylus cantonensis, isolated from different geographical regions revealed widespread multiple lineages2012

    • Author(s)
      Tokiwa T, et al
    • Journal Title

      Parasitol Int

      Volume: (in press) Issue: 3 Pages: 431-6

    • DOI

      10.1016/j.parint.2012.02.005

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 日本住血吸虫により分泌される細胞外小胞におけるmiRNAを介した雌雄コミュニケーションについて2015

    • Author(s)
      熊谷貴、市村浩一郎、山邊将史、下河原理江子、太田伸生
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 寄生扁形動物である日本住血吸虫における細胞外小胞の分泌を通したmiRNAコミュニケーション機構2014

    • Author(s)
      熊谷貴、市村浩一郎、沖野望、山邊将史、下河原理江子、関丈典、太田伸生
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Male-female interaction through miRNA contained in the extracellular vesicles secreted from Schistosoma japonicum (parasitic platehelminth)2014

    • Author(s)
      Kumagai T, Ichimura K, Yamabe M, Shimogawara R, Ohta N
    • Organizer
      Australasia Extracellular Vesicles Conference
    • Place of Presentation
      ケアンズ
    • Year and Date
      2014-11-20 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] The analysis of exosome-like vesicles from Schistosoma japonicum treated with erythrocytes2014

    • Author(s)
      Kumagai T, Ichimura K, Okino N, Yamabe M, Shimogawara R, Seki T, Ohta N
    • Organizer
      13th International Congress of Parasitology
    • Place of Presentation
      メキシコシティー
    • Year and Date
      2014-08-10 – 2014-08-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Efficacy analysis of newly synthesized compounds with endoperoxide activity, N-89/N-251, on larval stage Schistosoma mansoni2014

    • Author(s)
      Kumagai T, Yamabe M, Shimogawara R, Kim HS, Wataya Y, Ohta N
    • Organizer
      16th International Conference on Emerging Infectious Diseases in the Pacific Rim
    • Place of Presentation
      Dhaka, Bangladesh
    • Related Report
      2013 Research-status Report
  • [Presentation] 赤血球膜により刺激される日本住血吸虫のエクソソーム様小胞分泌に関する解析2014

    • Author(s)
      熊谷貴、市村浩一郎、沖野望、山邊将史、下河原理江子、関丈典、太田伸生
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      松山
    • Related Report
      2013 Research-status Report
  • [Presentation] Gene-knockdown by RNAi at the sporocyst stage of Schistosoma japonicum2013

    • Author(s)
      Kumagai T, Seki T, Yamabe M, Shimogawara R, Ohta N
    • Organizer
      Forum Cheju-16
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2013 Research-status Report
  • [Presentation] 日本住血吸虫スポロシスト期を標的としたRNAiによる遺伝子ノックダウン2013

    • Author(s)
      熊谷貴、山邊将史、下河原理江子、関丈典、太田伸生
    • Organizer
      第54回に本熱帯医学会大会
    • Place of Presentation
      長崎
    • Related Report
      2013 Research-status Report
  • [Presentation] 寄生性扁形動物である日本住血吸虫より分泌されるエクソソーム様小胞の免疫学的・生物学的機能の解析2013

    • Author(s)
      熊谷貴、山邊将史、下河原理江子、関丈典、太田伸生
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] Gene-knockdown against sporocysts from Schistosoma japonicum by RNAi2013

    • Author(s)
      Kumagai T, Yamabe M, Seki T, Shimogawara R, Ohta N.
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] The immunosuppressions by exosome/microvesicles secreted from the adult worms of Schistosoma japonicum.2012

    • Author(s)
      Kumagai T, Seki T, Taniguchi T, Shimogawara R, Ohta N.
    • Organizer
      The 15th Japan-Korea Parasitologist’ Seminar
    • Place of Presentation
      宮崎
    • Related Report
      2012 Research-status Report
  • [Presentation] 日本住血吸虫成虫より分泌されるExosomes/MicrovesiclesによるIFN-g産生の抑制2012

    • Author(s)
      熊谷貴、関丈典、谷口斎恵、下河原理江子、太田伸生
    • Organizer
      第23回日本生体防御学会学術総会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 日本住血吸虫から分泌されるエクソソーム様小胞による宿主免疫調節2012

    • Author(s)
      熊谷貴、関丈典、谷口斎恵、下河原理江子、太田伸生
    • Organizer
      第72回日本寄生虫学会東日本支部会・第10回分子寄生虫・マラリアフォーラム合同大会
    • Place of Presentation
      群馬
    • Related Report
      2012 Research-status Report
  • [Presentation] 寄生扁形動物である日本住血吸虫のsystemic RNAi deficiency-1(sid-1)遺伝子オルソログはdsRNAのトランスポート、及び、RNAiの機能に関与しない2012

    • Author(s)
      熊谷貴、山邊将史、沖野望、関丈典、下河原理江子、伊藤信、太田伸生
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Book] 寄生虫学研究 材料と方法 2014年版2014

    • Author(s)
      熊谷貴、下河原理江子、山邊将史、太田伸生
    • Total Pages
      179
    • Publisher
      三恵社
    • Related Report
      2014 Annual Research Report
  • [Remarks] 東京医科歯科大学国際環境寄生虫病学分野 研究概要

    • URL

      http://www.tmd.ac.jp/med/mzoo/kenkyu/saito/yan_jiu_gai_yao.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 東京医科歯科大学・国際環境寄生虫病学分野

    • URL

      http://www.tmd.ac.jp/med/mzoo/EnvPara_main.html

    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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