Analysis of unique lysosomal soluble protein transporting receptor family proteins in enteric protozoan parasite Entamoeba histolytica
| Project/Area Number |
24590513
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
TSUKUI Kumiko 国立感染症研究所, 寄生動物部, 主任研究官 (00420092)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | lysosome / receptor / traffic / Entamoeba histolytica / リソソーム / 赤痢アメーバ / 小胞輸送 / プロテアーゼ / 加水分解酵素 / アメーバ症 / アミラーゼ |
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| Outline of Final Research Achievements |
Lysosomal soluble proteins are targeted to endosomes and lysosomes by specific receptors. The enteric protozoan parasite Entamoeba histolytica has a novel class of lysosomal targeting receptors, named the cysteine protease binding protein family (CPBF). Among 11 CPBFs, CPBF1 is a solo receptor for cysteine proteases and other 6 CPBFs recognize glycolytic enzymes, i.e. amylases, β-hexosaminidases, and lysozymes. It was shown by bioinformatics analysis and phylogenetic reconstruction that each CPBF contains six prepeptidase carboxyl-terminal domains, and the domain configuration is evolutionarily conserved among CPBFs. Taken together, CPBFs with unique and conserved domain organisation have a remarkable ligand heterogeneity toward cysteine protease and carbohydrate degradation enzymes. These findings add new insights into the unique molecular event in enteric protozoan pathogen E. histolytica.
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Report
(4 results)
Research Products
(17 results)
