| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
I have studied the molecular mechanism by which Mycobacterium tuberculosis (MTB) stimulates or regulates the host immune response. In the series of experiments I found that the region of difference 1 (RD1), a genomic locus in the MTB genome that has been shown to participate in the virulence of the bacterium, contributes to the maturation and secretion of IL-1α by enhancing the level of intracellular Ca2+ followed by calpain activation. Furthermore, it has been shown that the inflammasome adaptor ASC is phosphorylated at a 144-tyrosine residue during inflammasome activation in a Syk- and Jnk-dependent manner. Phosphorylated ASC forms speck-like aggregate and contributes to caspase-1 activation. In addition, it was found that PD-1 inhibitory signal severely affects the function of Th1 cells and Tim3-dependent signal is critical to regulation of the differentiation of memory T cell into effector T cells.
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