Receptor of vibrioferrin, siderophore of Vibrio parahaemolyticus

Research Project

Project/Area Number	24590529
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Bacteriology (including Mycology)
Research Institution	University of the Ryukyus
Principal Investigator	Nakao Hiroshi 琉球大学, 医学部, 教授 (20237217)
Project Period (FY)	2012-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords	腸炎ビブリオ / 鉄 / siderophore / シデロフォア
Outline of Final Research Achievements	Vibrio parahaemolyticus requires iron for its survival and growth. V. parahaemolyticus produces siderophore, vibrioferrin (VF), under iron restriction conditions. Deletion mutants which lacks VF receptors were prepared, and showed that VF was necessary for growth of V. parahaemolyticus. Monoclonal antibodies against two VF receptors were prepared and showed binding ability with VF receptors. These clones may be useful for V. parahaemolyticus detection. Characterization of these antibodies are undergoing.