Mechanisms of higher order complex formation and Rab11-dependent apical transport of the segmented influenza virus genome
| Project/Area Number |
24590558
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | インフルエンザウイルス / RNP複合体 / 極性輸送 / Rab11 / ゲノム集合 |
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| Outline of Final Research Achievements |
Segmented genome of influenza A virus is thought to form a higher order complex by selective assembly of 8 different viral ribonucleoprotein complex (vRNP) before packaging. In this study, focusing on the mechanisms of vRNP trafficking and assembly in the infected cells, an experimental system for measuring the proximity of vRNP segments was established. By using this system, it was revealed that the selective segment assembly occurs most likely in the cytoplasm after the nuclear export of progeny vRNP. For this assembly process, the microtubule-dependent apical transport is not required.
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Report
(4 results)
Research Products
(7 results)
