| Project/Area Number |
24590667
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | Takasaki University of Health and Welfare |
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Principal Investigator |
OGIHARA Takuo 高崎健康福祉大学, 薬学部, 教授 (80448886)
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| Co-Investigator(Kenkyū-buntansha) |
KAORI Morimoto 高崎健康福祉大学, 薬学部, 講師 (90401009)
MASAHIRO Kajita 高崎健康福祉大学, 薬学部, 准教授 (40591871)
YOKO Idota 高崎健康福祉大学, 薬学部, 研究員 (90629594)
KENTARO Yano 高崎健康福祉大学, 薬学部, 助手 (40644290)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | P-糖タンパク / がん多剤耐性 / 裏打ちタンパク / 膜上発現 / 調節因子 / ＥＲＭタンパク / 組織選択性 / radixin / 多剤耐性 / 排出組織 / がん細胞 / 正常組織 / 輸送活性 / ラディキシン / 消化管吸収 / トランスポーター / 脳組織移行性 / シルニジピン / 脳保護作用 |
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| Outline of Final Research Achievements |
P-glycoprotein (P-gp) excretes many therapeutic drugs from the cytoplasm to outside the cells. Therefore, it is well known as a major factor in multidrug resistance of cancer cells. Our previous study demonstrated that radixin among ezrin, radixin and moesin (ERM proteins) regulated P-gp transport activity and membrane localization. In this study, we investigated the relationship between P-gp and ERM proteins in cancer cells from other organs. The transport activity of intestinal P-gp was regulated by radixin in both normal and cancer cells. On the other hand, it was suggested that none of the ERM proteins affected the renal P-gp in either normal or cancer cells. Thus, we hypothesize that the functional regulation mechanism of P-gp via ERM proteins varies between different organs, such as the intestine and kidney, but not between normal and cancer cells in the same organ.
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