Development of homogenous and quantitative immunoassay system with magnetic detection

• Project/Area Number: 24590716
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Nagasaki International University
• Principal Investigator: KUMA Hiroyuki 長崎国際大学, 薬学部, 准教授 (40435136)
• Co-Investigator(Kenkyū-buntansha): HAMASAKI Naotaka 長崎国際大学, 薬学部, 客員教授 (00091265)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 磁気的免疫検査システム / ホモジニアスアッセイ / 磁気マーカー / SQUID / 抗原抗体反応 / 医療・福祉 / 磁気検出 / ホモジニアス / 免疫検査

Outline of Final Research Achievements

In this study, we have developed the homogenous and quantitative immunoassay system with magnetic marker, polystyrene beads and SQUID sensor.
We also demonstrated the ability for magnetic markers to measure various biological targets (proteins, fugus, cedar pollen) without BF separation. This system has potential for use not only in clinical and laboratory immunoassays but also for medical inspections, identification of environmental pollution factors, and configuration of food examination tests.

Research Products

• [Journal Article] 国際的な標準化活動とJSCC (2014)
  • Author(s): 濱崎 直孝
  • Journal Title: 臨床検査 Volume: 58 Pages: 150-155
  • Peer Reviewed
• [Journal Article] 磁気検出法を用いた高速・高感度な免疫検査システム (2014)
  • Author(s): 隈 博幸
  • Journal Title: 生物化学的測定研究会年報 Volume: 18 Pages: 25-36
• [Journal Article] Activated protein C anticoagulant system dysfunction and thrombophilia in Asia (2013)
  • Author(s): Hamasaki N, Kuma H, Tsuda H.
  • Journal Title: Ann. Lab. Med. Volume: 33 Issue: 1 Pages: 8-13
  • DOI: 10.3343/alm.2013.33.1.8
  • Peer Reviewed
• [Journal Article] Beneficial effect of EPA (20:5 n-3 PUFA) on preventing venous thromboembolism: a rat tail thrombosis model experiment. (2013)
  • Author(s): Kuma H, Nagashima R, Hatae H, Tsuda T, Hamasaki N
  • Journal Title: Thromb Res Volume: 131(1) Issue: 1 Pages: 107-108
  • DOI: 10.1016/j.thromres.2012.09.014
  • Peer Reviewed
• [Journal Article] Characterization of magnetic markers for liquid-phase immunoassays using Brownian relaxation (2012)
  • Author(s): K.Enpuku, et al
  • Journal Title: Jpn.J.Appl.Phys. Volume: 51 Issue: 2R Pages: 23002-23002
  • DOI: 10.1143/jjap.51.023002
  • NAID: 40019170631
  • Peer Reviewed
• [Presentation] APC凝固制御異常と血栓性素因 (2014)
  • Author(s): 濱崎 直孝
  • Organizer: 第36回日本血栓止血学会プロテインS研究会シンポジウム
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2014-05-30
  • Invited
• [Presentation] Homogenous quantitative analysis of human parathyroid hormone by use of magnetic markers and (2013)
  • Author(s): Maeda C, Hisamatsu K, Mitsuse, K, Egashira, S,
  • Organizer: IFCC EuroMedlab 2013
  • Place of Presentation: イタリア・ミラノ
• [Presentation] Homogenous quantitative analysis of human parathyroid hormone by use of magnetic markers and SQUID magnetometer (2013)
  • Author(s): Maeda C, Hisamatsu K, Mitsuse, K, Egashira, S, Hamasaki, N, Enpuku, K, Kuma, H
  • Organizer: International Federation of Clinical Chemistry
  • Place of Presentation: イタリア・ミラノ
• [Presentation] 磁気検出法を用いた高速・高感度な免疫検査システム
  • Author(s): 隈 博幸
  • Organizer: 第18回生物化学的測定研究会学術集会
  • Place of Presentation: キャンパスプラザ京都
  • Invited
• [Remarks] 長崎国際大学薬学部臨床検査学研究室HP（磁気検出法を用いた新規免疫検査システムの開発）
  • URL: http://www.niu.ac.jp/~pharm1/lab/cclm/immunoassay.html
• [Remarks] 長崎国際大学薬学部臨床検査学研究室ホームページ
  • URL: http://www.niu.ac.jp/~pharm1/lab/cclm/immunoassay.html