Development of homogeneous assay for HDL cholesterol subfractions and assessment for clinical application
| Project/Area Number |
24590717
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kumamoto Health Science University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松嶋 和美 熊本保健科学大学, 保健科学部, 講師 (00369125)
安東 由喜雄 熊本大学, その他の研究科, 教授 (20253742)
安楽 健作 熊本保健科学大学, 保健科学部, 講師 (80389543)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Keywords | コレステロール / HDL亜分画コレステロール / ホモジニアス法 / HDL2-コレステロール / HDL3-コレステロール / HDL亜分画 / HDL2-C / HDL3-C / HDL2 / HDL3 |
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| Outline of Final Research Achievements |
In this study, we found that addition of the cationic surfactant and a polyethylene glycol modified enzyme to the cholesterol assay system selectively solubilizes HDL3-C. We then applied this knowledge to the development of a homogeneous HDL3-C assay that is simple and requires no separation processing. The repeatability of this method was found to be 2.0% or less by CV% for HDL3-C in the range of 10 to 30 mg/dl, and its coefficient of correlation with the subfraction by ultracentrifugation was r=0.841 with the regression equation y=0.884x＋4.807 for n=20. The method can be embodied in an automatic analyzer for simple, fast measurement of HDL3-C using a minute amount of sample, and is expected to substantially contribute to the treatment and prevention of arteriosclerotic disease.
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Report
(4 results)
Research Products
(16 results)
