Autopsy diagnosis of deaths due to overdoses of insulin: development of detection methods of insulin formulations

Research Project

Project/Area Number	24590873
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Legal medicine
Research Institution	Fukuoka University
Principal Investigator	KASHIWAGI Masayuki 福岡大学, 医学部, 准教授 (70301687)
Co-Investigator(Kenkyū-buntansha)	HARA Kenji 福岡大学, 医学部, 講師 (00090738) WATERS Brian 福岡大学, 医学部, 助教 (00609480) KUBO Shin-ichi 福岡大学, 医学部, 教授 (10205122)
Project Period (FY)	2012-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	インスリン / GC-MS / LC-MS/MS / 低血糖症 / GC/MS / LC/MS/MS
Outline of Final Research Achievements	During this study period, meta-cresol, one of the additives in insulin formulations, could be detected with a head space-solid phase micro extraction (HS-SPME) method using gas chromatography-tandem mass spectrometry (GC-MS/MS), and quantitation was believed to be achievable by the application of the method. For insulin analogues, detection was possible using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and quantitation was also considered a possibility.