| Project/Area Number |
24591319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Okinaka Memorial Institute for Medical Research (2014)
University of Yamanashi (2012-2013) |
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Principal Investigator |
KOBAYASHI Tetsuro 公益財団法人冲中記念成人病研究所, その他部局等, 研究員 (30113442)
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| Co-Investigator(Kenkyū-buntansha) |
AIDA Kaoru 山梨大学, 総合研究部, 准教授 (50184015)
ICHIJO Masashi 山梨大学, 総合研究部, 助教 (50436854)
TAKIZAWA Souichi 山梨大学, 総合研究部, 助教 (80456467)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 糖尿病 / 緩徐1型糖尿病 / NODマウス / CD28 / 緩徐進行1型糖尿病 / 1型糖尿病 / Type 1 diabetes / Innate immunity / ER stress |
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| Outline of Final Research Achievements |
The characteristic pathological features of autopsied pancreas of SPIDDM were CD8+ T cell and CD68+ macropthages to the atrophied exocrine pancreas. Sixty percent of SPIDDM pancreas had pancreatic ductal hyperplasia/metaplasia with obstructive pancreatitis (p<0.01 vs. non-diabetic controls). To establish an animal model of SPIDDM, the effect of poly I:C multiple intraperitoneal injection (poly I:C model) or intra-pancreatic ductal injection of Zein-oleic acid-linoleic acids (ZOAL) (ZOAL model) to CD28 knock-out NOD mice was examined. ZOAL model showed marked pancreatic exocrine inflammation composed of CD8+ and CD68+ cells and high occurrence of diabetes, while poly I:C model showed slight exocrine pancreatic inflammation without enhanced occurrence of diabetes.
Conclusion: We could established ZOAL NOD mice model, which resembles with human SPIDDM.
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