Glucose regulates the function of glucocorticoid receptor via O-GlcNAc modification.
| Project/Area Number |
24591357
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | The University of Tokushima (2013-2014)
Gunma University (2012) |
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Principal Investigator |
SAWATSUBASHI Shun 徳島大学, 藤井節郎記念医科学センター, 特任講師 (70535103)
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| Co-Investigator(Renkei-kenkyūsha) |
KITAGAWA Hirochika 群馬大学, 生体調節研究所, 教授 (20345234)
SATO Takashi 群馬大学, 生体調節研究所, 准教授 (70344934)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | グルココルチコイド / 翻訳後修飾 / O-GlcNAc / 核内受容体 / ステロイドホルモン / グルココルチコイドレセプター / GR / O-GlcNAc修飾 / OGT / グルココルチコイド受容体 / O-GlcNAc転移酵素 / グルコース / リン酸化 / 転写 |
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| Outline of Final Research Achievements |
Glucocorticoids are widely used as anti-inflammatory drugs, the molecular mechanisms involved in this anti-inflammatory effect and associated with serious side effects remain unknown. In this study, we performed the proteomic analysis of post-translational modification on glucocorticoid receptor (GR) and identified a novel O-linked beta-N-acetylglucosamine (O-GlcNAc) modified sites. Moreover, this O-GlcNAcylation was regulated under conditions of glucose concentration in culture medium and detected in nucleus of mouse liver and cerebrum. Based on these results, we propose that this O-GlcNAcylation regulates the tissue-specific transcriptional activity of GR.
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Report
(4 results)
Research Products
(3 results)
