Identification of novel proteases involved in the regulation of highly pathogenic avian influenza (HPAI) viruses infection
| Project/Area Number |
24591481
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Infectious disease medicine
|
|---|
| Research Institution | Sagami Women's University (2013-2014)
The University of Tokushima (2012) |
|---|
Principal Investigator |
YUUSHI Okumura 相模女子大学, 公私立大学の部局等, 教授 (70294725)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NIKAWA Takeshi 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (20263824)
HIRASAKA Katsuya 長崎大学, 水産学部, 助教 (70432747)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 高病原性鳥インフルエンザウイルス / ウイルス活性化酵素 / 膜結合型プロテアーゼ / プロテアーゼ阻害剤 |
|---|
| Outline of Final Research Achievements |
Cleavage of viral envelope glycoprotein, hemagglutinin (HA), by host cellular proteases is essential step for influenza virus to enter into the target cells. We identified that ubiquitous type II transmembrane serine proteases, MSPL and its splice variant TMPRSS13, were candidates of HA-processing proteases of diverse highly pathogenic avian influenza (HPAI) viruses. To confirm the involvement of these proteases in HPAI virus infection, highly virulent virus (A/Crow/Kyoto/53/2004 (H5N1)) was infected into MSPL/TMPRSS13 stably expressed cells and MSPL/TMPRSS13-deficient mice (MSPL/TMPRSS13-/-). As a result, we concluded that these proteases productions might be responsible for HPAI virus multicycle replication and spreading. We next succeeded to reveal the crystal structure of MSPL/TMPRSS13. Based on their structure, we generated specific inhibitors for MSPL/TMPRSS13. In vivo infectious experiments using their specific inhibitors are currently being investigated.
|
Report
(4 results)
Research Products
(18 results)
-
[Journal Article] A novel myogenic function residing in the 5’ non-coding region of Insulin receptor substrate-1 (Irs-1) transcript.2015
Author(s)
Nagano H, Yamagishi N, Tomida C, Yano C, Aibara K, Kohno S, Abe T, Ohno A, Hirasaka K, Okumura Y, Mills EM, Nikawa T, Teshima-Kondo S.
-
Journal Title
BMC Cell Biol
Volume: in press
Issue: 1
Pages: 1-10
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
-
[Journal Article] Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle Atrophy -Associated Ubiquitin Ligase Cbl-b2013
Author(s)
Abe T, Kohno S, Yama T, Ochi A, Suto T, Hirasaka K, Ohno A, Teshima-Kondo S, Okumura Y, Oarada M, Choi I, Mukai R, Terao J, Nikawa T
-
Journal Title
Int J Endocrinol
Volume: 2013
Pages: 907565-907565
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
