Elucidation of pathologic conditions and establishment of the diagnostic methods in hydrolyzed wheat protein (Glupearl: GP19S)- induced immediate-type wheat allergy.
Project/Area Number |
24591671
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Dermatology
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Research Institution | Fujita Health University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
YAGAMI Akiko 藤田保健衛生大学, 医学部, 准教授 (90367699)
SANO Akiyo 藤田保健衛生大学, 医学部, 助教 (20625983)
NAKAMURA Masashi 藤田保健衛生大学大学院, 医学研究科 (30449467)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | 加水分解コムギ / グルパール19S / 即時型コムギアレルギー / 特異IgE抗体 / 検査法 / 抗原解析 / 交叉反応性 / 予後因子の探索 / グルパール19S / 経皮膚・粘膜感作 / 小麦アレルギー / 交差反応性 / 診断方法の構築 / 予後因子探索 / 重症度分類 / ELISA法 |
Outline of Final Research Achievements |
The purpose of this study was to elucidate the pathologic conditions and to establish the diagnostic methods in hydrolyzed wheat protein- induced immediate-type wheat allergy (HWP-IWA) . Our findings show that the ELISA-based GP19S specific IgE assay is a useful quantitative and high throughput method for the diagnosis. Using ELISA, skin prick test and Western blotting, we elucidated that patients who developed an allergy to GP19S also had cross-reactivity to some other HWPs. This suggested that other HWPs might contain the epitope sequences in GP19S. On the other hands, the GP19S antigen was thought to be generated after gluten was exposed to hydrochloric acid and heated to 95 °C. The heating time was long enough to initiate deamidation but too short to initiate a change in molecular weight, and precipitation at the isoelectric point resulted in an increase in antigen concentration.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] A New Reliable Method for Detecting Specific IgE Antibodies in the Patients with Immediate Type Wheat Allergy due to Hydrolyzed Wheat Protein: Correlation of Its Titer and Clinical Severity2014
Author(s)
Nakamura M., Yagami A.,Hara K., Sano A., Kobayashi T., Aihara M., Hide M.,Chinuki Y., Morita E., Teshima R., Matsunaga K
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Journal Title
Allergology International
Volume: 63
Issue: 2
Pages: 243-249
DOI
NAID
ISSN
1323-8930, 1440-1592
Related Report
Peer Reviewed / Open Access
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[Journal Article] Epithelial-mesenchymal transition of the eccrine glands is involved in skin fibrosis in morphea2013
Author(s)
Masayuki Takahashi, Hirohiko Akamatsu, Akiko Yagami, Seiji Hasegawa, Shiroh Ohgo, Masamichi Abe, Yohei Iwata, Masaru Arima, Hiroshi Mizutani, Satoru Nakata, Kayoko Matsunaga
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Journal Title
Journal of Dermatology
Volume: 40
Pages: 720-725
Related Report
Peer Reviewed
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[Journal Article] ジャパニーズスタンダードアレルゲン(1994)の2005年度~2007年度陽性率とジャパニーズスタンダードアレルゲン(2008)の2009年度陽性率2012
Author(s)
鈴木加余子,松永佳世子,矢上晶子,足立厚子,伊藤正俊,乾重樹,宇宿一成,海老原全,大磯直毅,岡惠子,河合敬一,鹿庭正昭,関東裕美,佐々木和実,杉浦真理子,杉山真理子,大迫順子,高山かおる,角田孝彦,尾藤利憲 他
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Journal Title
Journal of Environmental Dermatology and Cutaneous Allergology
Volume: 6(2)
Pages: 67-84
NAID
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[Presentation] linical analysis of imme-diate hypersensitivity to hydrolyzed wheat proteins in soap.2015
Author(s)
Yagami A, Suzuki K, Nakamura M, Sano A, Arima M, Iwata Y, Kobayashi T, Hara K, Matsunaga K
Organizer
American Association of Asthma, Allergy and Immunology Annual Meeting 2015
Place of Presentation
アメリカ,ヒューストン,George R. Brown Convention Center
Year and Date
2015-02-20 – 2015-02-24
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