The association between chorein and mitochondria-related cell death

• Project/Area Number: 24591685
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: Kagoshima University
• Principal Investigator: NAKAMURA Masayuki 鹿児島大学, 医歯(薬)学総合研究科, 准教授 (90332832)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 有棘赤血球舞踏病 / chorein / オートファジー / マイトファジー / mitophagy / autophagy / beta-adducin

Outline of Final Research Achievements

Co-immunoprecipitation assay and immunocytochemical analysis using chorein-overexpressing cells showed interaction between chorein and cytoskeltal proteins and autophagy-related proteins. The chorein-cytosleltal proteins iteraction was increased in CCCP-treated cells. Cell viability was significantly increased in chorein-overexpressing cells compared with mock transfected control cells during nutrient deprivation and during CCCP treatment. These results suggest that chorein interacts with cytoskeltal proteins and autophagy-related proteins and may play protective roles in cell death mediated by autophagy- and mitophagy-related mechanisms. Their disruption may be one of the molecular pathogenic mechanisms of chorea-acanthocytosis.

Research Products

• [Journal Article] Chorein, the proTein responsible for chorea-acanthocytosis, interacts with β-adducin and β-actin (2013)
  • Author(s): Shiokawa N, Nakamura M, SameShima M, Deguchi A, Hayashi T, Sasaki N, Sano A
  • Journal Title: Biochem Biophys res Commun Volume: 441 Issue: 1 Pages: 96-101
  • DOI: 10.1016/j.bbrc.2013.10.011
  • Peer Reviewed
• [Presentation] Chorein interacts with α-tubulin and HDAC6, and its overexpression preserves cell viability from nutrient starvation. (2014)
  • Author(s): Natsuki Sasaki, Masayuki Nakamura, Nari Shiokawa, Akiko Kodama, Akira Sano
  • Organizer: 第57回日本神経化学会2014
  • Place of Presentation: 奈良（奈良県文化会館）
  • Year and Date: 2014-09-29 – 2014-10-01
• [Presentation] Modifiers for symptoms of chorea-acanthocytosis -Effect of genetic background on ChAc model mouse (2014)
  • Author(s): Hitoshi Sakimoto, Omi Nagata, Masayuki Nakamura and Akira Sano
  • Organizer: 第36回生物学的精神医学会
  • Place of Presentation: 奈良（奈良県文化会館）
  • Year and Date: 2014-09-29 – 2014-10-01
• [Presentation] Identification of binding partners of chorein (2013)
  • Author(s): 塩川奈理, 中村雅之, 出口晃子, 佐々木なつき, 佐野 輝
  • Organizer: Neuro 2013
  • Place of Presentation: 京都
• [Presentation] Chorein interacts with cytoskeletal proteins on nutrient starvation in HEK293 cells (2013)
  • Author(s): 佐々木なつき, 中村雅之, 塩川奈理, 出口晃子, 佐野輝
  • Organizer: Neuro 2013
  • Place of Presentation: 京都
• [Presentation] Chorein interacts with cytoskeltal proteins in HEK293 cells (2012)
  • Author(s): Akiko Deguchi, Masayuki Nakamura, Nari Shiokawa, Natsuki Sasaki, Akira Sano
  • Organizer: The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry / The 55th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: 神戸
• [Book] 神経症候群（第２版）-その他の神経疾患を含めて- II章 III 変性疾患 錐体外路系疾患 不随意運動を主とする疾患 舞踏運動症候群 「有棘赤血球舞踏病(Chorea-acanthocytosis)」 (2014)
  • Author(s): 中村雅之、佐野 輝
  • Total Pages: 5
  • Publisher: 日本臨床社
• [Book] 脳とこころのプライマリケア2巻 知能と衰え VII章 認知症各論 (Huntington病, 歯状核赤核淡蒼球ルイ体萎縮症, 有棘赤血球舞踏病) (2013)
  • Author(s): 中村雅之、佐野 輝
  • Total Pages: 7
  • Publisher: シナジー