The association between chorein and mitochondria-related cell death
| Project/Area Number |
24591685
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Kagoshima University |
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Principal Investigator |
NAKAMURA Masayuki 鹿児島大学, 医歯(薬)学総合研究科, 准教授 (90332832)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 有棘赤血球舞踏病 / chorein / オートファジー / マイトファジー / mitophagy / autophagy / beta-adducin |
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| Outline of Final Research Achievements |
Co-immunoprecipitation assay and immunocytochemical analysis using chorein-overexpressing cells showed interaction between chorein and cytoskeltal proteins and autophagy-related proteins. The chorein-cytosleltal proteins iteraction was increased in CCCP-treated cells. Cell viability was significantly increased in chorein-overexpressing cells compared with mock transfected control cells during nutrient deprivation and during CCCP treatment. These results suggest that chorein interacts with cytoskeltal proteins and autophagy-related proteins and may play protective roles in cell death mediated by autophagy- and mitophagy-related mechanisms. Their disruption may be one of the molecular pathogenic mechanisms of chorea-acanthocytosis.
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Report
(4 results)
Research Products
(8 results)
