Fundamental analyses of our near-infrared fluorescent liposome system as therapeutic agents against gastrointestinal malignancies

• Project/Area Number: 24591933
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Chiba University
• Principal Investigator: HAYASHI Hideki 千葉大学, フロンティア医工学センター, 教授 (20312960)
• Co-Investigator(Renkei-kenkyūsha): TOYOTA Taro 東京大学, 総合文化研究科, 准教授 (80422377) ; TAMURA Yutaka 千葉大学, 大学院医学研究院, 准教授 (50263174)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: インドシアニングリーン / 光線温熱療法 / 光線力学療法 / ハプテン

Outline of Final Research Achievements

We tested the basic effectiveness of our near-infrared fluorescent (NIRF) liposome system with newly-made hydrophobic form of Indocyanine green (ICG-C18) in gastrointestinal cancer therapy using tumor bearing mouse model. When NIRF liposomes of 200nm in mean diameter were administered to mice inoculated with esophageal carcinoma cell line, the liposomes were revealed to accumulate in the tumor with EPR effect. NIR light irradiation to the tumor after administration of the liposomes showed significant suppression of the tumor growth. Administration of the liposomes containing cisplatin indicated enhanced tumor growth suppression. In vitro analysis of the liposomes containing Dinitrophenol revealed that 1.5 to 4 % of the contents were released after irradiation of NIR light.

Research Products

• [Journal Article] Treatment of near-infrared photodynamic therapy using a liposomally formulated indocyanine green derivative for squamous cell carcinoma (2015)
  • Author(s): Maruyama T, Akutsu Y, Suganami A, Tamura Y, Fujito H, Ouchi T, Akanuma N, Isozaki Y, Takeshita N, Hoshino I, Uesato M, Toyota T, Hayashi H, Matsubara H
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0122849-e0122849
  • DOI: 10.1371/journal.pone.0122849
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Detection of Peritoneal Dissemination with Near-infrared Fluorescence Laparoscopic Imaging Using a Liposomal Formulation of a Synthesized Indocyanine Green Liposomal Derivative (2015)
  • Author(s): Hoshino I, Maruyama T, Fujito H, Tamura Y, Suganami A, Hayashi H, Toyota T, Akutsu Y, Murakami K, Isozaki Y, Akanuma N, Takeshita N, Toyozumi T, Komatsu A, Matsubara H
  • Journal Title: Anticancer research Volume: 35 Pages: 1353-1359
  • Peer Reviewed
• [Journal Article] 【獣医学におけるレーザー診療】 ICG-Lipoの化学的・生物学的特性 (2014)
  • Author(s): 菅波晃子, 豊田太郎, 岡崎茂俊, 林 秀樹, 岡本芳晴, 田村 裕
  • Journal Title: 日本レーザー医学会誌 Volume: 35 Pages: 39-45
• [Journal Article] Near-infrared-fluorescence imaging of lymph nodes by using liposomally formulated indocyanine green derivatives (2014)
  • Author(s): Taro Toyota, Hiromichi Fujito, Akiko Suganami, Tomoki Ouchi, Aki Ooishi, Akira Aoki, Kazutaka Onoue, Yutaka Muraki, Tomoyuki Madono, Masanori Fujinami, Yutaka Tamura, Hideki Hayashi
  • Journal Title: Bioorganic & Medical Chemistry Volume: 22 Issue: 2 Pages: 721-727
  • DOI: 10.1016/j.bmc.2013.12.026
  • Peer Reviewed / Open Access
• [Journal Article] Development of a non-blurring, dual-imaging tissue marker for gastrointestinal tumor localization (2014)
  • Author(s): Hayashi, H., Toyota, T., Goto, S., Ooishi, A., Gao, T., Ee, L. B., Hatayama, H., Nomoto, T., Fujinami, M, Matsubara, H
  • Journal Title: Surg Endosc Volume: - Issue: 6 Pages: 1445-1451
  • DOI: 10.1007/s00464-014-3822-1
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Application of a Novel Near Infrared-Fluorescence Giant Vesicle- and Polymerasome-based Tissue Marker for Endoscopic and Laparoscopic Navigation (2014)
  • Author(s): Hirosuke Hatayama, Taro Toyota, Hideki Hayashi, Tomonori Nomoto, Masanori Fujinami
  • Journal Title: Analytical Sciences Volume: 30 Issue: 2 Pages: 225-230
  • DOI: 10.2116/analsci.30.225
  • NAID: 130004442257
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 安全で質の高い低侵襲化外科治療をもたらす近赤外蛍光イメージング (2013)
  • Author(s): 林 秀樹，豊田太郎，田村 裕，松原久裕
  • Journal Title: 電子情報通信学会技術研究報告 Volume: MI2013-44 Pages: 35-40
• [Journal Article] 新しい近赤外蛍光色素を用いた消化管腫瘍に対する手術ナビゲーションの開発 (2012)
  • Author(s): 林秀樹，田村裕，豊田太郎，真殿智行，大内友貴，後藤翔一，松原久裕．
  • Journal Title: 映像情報メディカル Volume: 44 Pages: 503-509
• [Presentation] 疎水性インドシアニングリーン誘導体のセンチネルリンパ節同定における有用性の検討 (2014)
  • Author(s): 章 逸汀，矢作亮介，大友修平，高 涛，Lau Bik Ee，豊田太郎，林 秀樹，松原久裕
  • Organizer: 第7回蛍光Navigation Surgery研究会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-18
• [Presentation] Near-infrared fluorescence imaging with liposomal formulation of an indocyanine green derivative for laparoscopic detection of sentinel lymph nodes (2014)
  • Author(s): Hideki Hayashi, Taro Toyota, Yutaka Tamura, Tomoyuki Madono, Hiromichi Fujito, Masanori Fujinami, Hisahiro Matsubara
  • Organizer: 14th World Congress of Endoscopic Surgery
  • Place of Presentation: Paris, France
  • Year and Date: 2014-06-24 – 2014-06-28
• [Presentation] Development of a non-blurring dual-imaging (X-ray/fluorescence) tissue marker for laparoscopic treatment of gastric cancer (2014)
  • Author(s): Hayashi H, Toyota T, Ooishi A, Gao T, Lau BE, Matsubara H.
  • Organizer: 13th Annual Surgery of the Foregut Symposium
  • Place of Presentation: Coral Gables, Florida, USA
  • Invited
• [Presentation] Laparoscopic Resection of Gastric Cancer: The Japanese Experience (2013)
  • Author(s): Hayashi H, Oyama Y, Kameda N.
  • Organizer: St. John Providence Health System GI Cancer Symposium 2013
  • Place of Presentation: Detroit, USA
  • Invited
• [Presentation] 安全で質の高い低侵襲化外科治療をもたらす近赤外蛍光イメージング (2013)
  • Author(s): 林 秀樹，豊田太郎，田村 裕，松原久裕
  • Organizer: 2013年度 医用画像研究会 9月研究会
  • Place of Presentation: 千葉
  • Invited
• [Presentation] センチネルリンパ節同定のための新しい近赤外リポソームの開発 (2013)
  • Author(s): 大石 知，豊田太郎，田村 裕，高 涛，LAU BIK EE，林 秀樹
  • Organizer: 第6回蛍光Navigation Surgery研究会
  • Place of Presentation: 神戸
• [Presentation] 近赤外蛍光化リポソームを用いたセンチネルリンパ節同定法の開発 (2013)
  • Author(s): 林 秀樹，大石 知，豊田太郎，田村 裕，高 涛，LAU BIK EE，松原久裕
  • Organizer: 第15回SNNS研究会
  • Place of Presentation: 釧路
• [Presentation] Development of a non-blurring, dual-imaging (X-ray/fluorescence) tissue marker for tumor localization ahead of and during laparoscopic gastrectomy
  • Author(s): Hideki Hayashi, Taro Toyota, Kohsuke Tajima, Shouichi Goto, Terumasa Yamaoka, Gao Tao, Hisahiro Matsubara.
  • Organizer: 20th International Congress of the European Association for Endoscopic Surgery
  • Place of Presentation: Brussels, Belgium
• [Presentation] ナノ粒子ICG-Liposposomeと光学力学的による扁平上皮癌に対する温熱療法の開発
  • Author(s): 丸山哲郎，阿久津泰典，星野敢，森幹人，羽成直行，藤戸廣迪，菅波晃子，田村裕，林秀樹，松原久裕
  • Organizer: 第66回日本食道学会学術集会
  • Place of Presentation: 長野
• [Presentation] 近赤外蛍光プローブを用いた消化管腫瘍に対するナビゲーションサージャリー
  • Author(s): 林秀樹，藤戸寛迪，豊田太郎，田村裕，真殿智行，松原久裕．
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 千葉
  • Invited
• [Presentation] 独自に開発したICG-Liposomeによる扁平上皮癌に対する光線力学的温熱療法の開発
  • Author(s): 丸山哲郎，阿久津泰典，星野敢，森幹人，羽成直行，藤戸寛迪，菅波晃子，田村裕，林秀樹，松原久裕
  • Organizer: 第67回日本消化器外科学会総会
  • Place of Presentation: 富山
• [Presentation] 新規蛍光色素を用いた低拡散性近赤外蛍光・X線デュアルイメージング組織マーカーの開発
  • Author(s): 後藤翔一，大内友貴，豊田太郎，田村 裕，真殿智行，松原久裕，林 秀樹
  • Organizer: 第5回蛍光Navigation Surgery研究会
  • Place of Presentation: 京都
• [Book] Concepts and Applications of Fluorescence Imaging for Surgeons (2015)
  • Author(s): Fernando Dip and Takeshi Ishizawa
  • Publisher: Springer
• [Remarks] 千葉大学大学院工学研究科人工システム科学専攻メディカスシステムコース研究室紹介
  • URL: http://www.tms.chiba-u.jp/div.html
• [Patent(Industrial Property Rights)] LIPOSOME COMPOSITE BODY (2014)
  • Inventor(s): 鳥取大学、千葉大学
  • Industrial Property Rights Holder: 鳥取大学、千葉大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-05-19
  • Overseas