Modeling the development of notochord and floor plate with human induced pluripotent stem cells.
Project/Area Number |
24592124
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cerebral neurosurgery
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Research Institution | Kyoto University |
Principal Investigator |
MORIZANE Asuka 京都大学, iPS細胞研究所, 助教 (10528730)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 多能性幹細胞 / 脊索 / 神経 / 分化誘導 |
Outline of Final Research Achievements |
Notochord appears in the developing embryo close to the neural tube. It secretes Sonic hedgehog (Shh) and Noggin that induce specific of neurons such as dopaminergic neurons.The research of notochord has been proceeded with Xenopus or Chick. Regarding human, as it is difficult to use its embryo, the notochord of human development has not been studied. In this study, human pluripotent stem cells made it possible to generate the in vitro model of development of human notochord and neural tube. Many combinations of known factors and small molecules has been tried to induce human notochord from pluripotent stem cells. Finally the markers expressed in notochord such as Shh and Foxa2 were observed in the induced cells. This invented protocol enables the study of notochord development with human cells that has been difficult without pluripotent stem cells.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.2014
Author(s)
Masato Nakagawa, Yukimasa Taniguchi, Sho Senda, Nanako Takizawa, Tomoko Ichisaka, Kanako Asano, Asuka Morizane, Daisuke Doi, Jun Takahashi, Masatoshi Nishizawa, Yoshinori Yoshida, Taro Toyoda, Kenji Osafune, Kiyotoshi Sekiguchi and Shinya Yamanaka
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Journal Title
Sci Rep
Volume: 4
Pages: 3594
DOI
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[Journal Article] Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells.2013
Author(s)
Michiyo Koyanagi-Aoi, Mari Ohnuki, Kazutoshi Takahashi, Keisuke Okita, Hisashi Noma, Yuka Sawamura, Ito Teramoto, Megumi Narita, Yoshiko Sato, Tomoko Ichisaka, Naoki Amano, Akira Watanabe, Asuka Morizane, Yasuhiro Yamada, Tosiya Sato, Jun Takahashi and Shinya Yamanaka
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Journal Title
Proc Natl Acad Sci U S A
Volume: 110
Pages: 20569-74
DOI
Related Report
Peer Reviewed
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[Presentation] Autologous Transplantation with iPSC-derived Neurons is Immunologically Beneficial even in the Brain2014
Author(s)
Asuka Morizane, Daisuke Doi, Tetsuhiro Kikuchi, Keisuke Okita, Akitsu Hotta, Toshiyuki Kawasaki, Hirotaka Onoe, Takashi Shiina , Shinya Yamanaka, and Jun Takahashi
Organizer
The 7th Takeda Science Foundation Symposium on PharmaSciences,
Place of Presentation
Osaka, Japan
Year and Date
20140116-20140118
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