| Project/Area Number |
24592204
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
TANABE Fumito 鹿児島大学, 医歯(薬)学総合研究科, 助教 (90619199)
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| Co-Investigator(Kenkyū-buntansha) |
KOMIYA Setsuro 鹿児島大学, 大学院医歯学総合研究科, 教授 (30178371)
SETOGUCHI Takao 鹿児島大学, 大学院医歯学総合研究科, 特任准教授 (40423727)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 神経幹細胞 / iPS細胞 / オートファジー / 低酸素 / 脊髄損傷 / 慢性圧迫性脊髄障害 |
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| Outline of Final Research Achievements |
We used the neural stem cell cell line AF22 which is derived by human iPS cell.The expression of LC3-II, which is a marker of autophagy, was upregulated in 1%O2 condition compared to 20% O2condition. LiCl which is an autophagy activator increased the proliferation of AF22. In addition, rapamycin which is an another autophagy activator increased the proliferation of AF22 under 1% O2 and 20% O2 conditions. Furthermore, differentiation capacity of AF22 was not chaged under 1% O2 condition.
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