Practical use with control of aquaporin for brain edema decrease using knockdown technique

• Project/Area Number: 24592310
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Aichi Medical University (2014); Nagoya City University (2012-2013)
• Principal Investigator: FUJITA Yoshihito 愛知医科大学, 医学部, 教授 (90238593)
• Co-Investigator(Kenkyū-buntansha): SOBUE Kazuya 名古屋市立大学大学院, 医学研究科, 教授 (90264738) ; ASAI Kiyofumi 名古屋市立大学大学院, 医学研究科, 教授 (70212462)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: aquaporin / knockdown / hypothermia / アクアポリン / 脳浮腫 / ノックダウン / AQP4

Outline of Final Research Achievements

Using vector expressing shRNA, we constructed lentivirus expressing sh RNAiof aquaporin(AQP) 4. We confirmed AQP4 knockdown that was stronger than our earlier study. We constructed hAQP4-M23 and hAQP4-M1 vector and transfected into C6 cells. We confirmed the overexpression of hAQP4-M1 and hAQP4-M23 in C6 cells. Moreover, we constructed permanent cell line of hAQP4-M23-overexpressed-cells of C6.

Research Products

• [Journal Article] Estimation of the success rate of anesthetic management for thymectomy in patients with myasthenia gravis treated without muscle relaxants: A retrospective observational cohort study (2015)
  • Author(s): Yoshihito Fujita, Satoru Moriyama, Satoshi Aoki, Saya Yoshizawa, Maiko Tomita, Taiki Kojima, Yukiko Mori, Naoko Takeuchi, Min-Hye So, Motoki Yano, Kazuya Sobue.
  • Journal Title: Jounal of Anesthesia Volume: 29 Pages: 1-1
  • Peer Reviewed
• [Journal Article] Leak in PerseusTM workstation after complete check sequences (2015)
  • Author(s): Hiroyuki Kinosshita, Noboru Hatakeyama, Yoshihito Fujita, Yoshihiro Fujiwara
  • Journal Title: Journal of Clinical Anesthesia Volume: 27 Pages: 1-1
  • Peer Reviewed
• [Journal Article] 大学病院ICUにおける最近5年間の小児veno-arterial extracorporeal membrane oxygenationの稼働実績と今後の課題 (2015)
  • Author(s): 仙頭佳起、竹内直子、太田晴子、佐野文昭、上村友二、藤田義人、祖父江和哉
  • Journal Title: 日本集中治療医学会雑誌 Volume: 22 Pages: 1-1
  • NAID: 130005107910
  • Peer Reviewed
• [Journal Article] Clinical usefulness of blood lactate level as a prognostic indicator comparing with Acute Physiology and Chronic Health Evaluation (APACHE) II scores: A retrospective, observational, cohort study (2014)
  • Author(s): Yoshihito Fujita, Tae Kato, Hiroki Yamauchi, Eisuke Kako, Min-Hye So, Tetsuya Tamura, Kazuya Sobue
  • Journal Title: Nagoya Medical Journal Volume: 59 Pages: 1-1
  • Peer Reviewed
• [Journal Article] Markedly elevated procalcitonin in early postoperative period in pediatric open heart surgery: a prospective cohort study (2014)
  • Author(s): Minami E, Ito S, Sugiura T, Fujita Y, Sasano H, Sobue K.
  • Journal Title: Journal of Intensive Care Volume: 2 Issue: 1 Pages: 38-38
  • DOI: 10.1186/2052-0492-2-38
  • Peer Reviewed / Open Access
• [Journal Article] Tidal volume and airway pressure under percutaneous transtracheal ventilation without a jet ventilator: comparison of high-flow oxygen ventilation and manual ventilation in complete and incomplete upper airway obstruction models. (2013)
  • Author(s): Sasano N, Tanaka A, Muramatsu A, Fujita Y, Ito S, Sasano H, Sobue K
  • Journal Title: Journal of Anesthesia Volume: E pub Issue: 3 Pages: 341-6
  • DOI: 10.1007/s00540-013-1733-2
  • Peer Reviewed
• [Journal Article] A comparison between dosages and plasma concentrations of dexmedetomidine in clinically ill patients: a prospective, observational, cohort study in Japan (2013)
  • Author(s): Yoshihito Fujita, Koichi Inoue, Tasuku Sakamoto, Saya Yoshizawa, Maiko Tomita, Yoshie Maeda, Hitomi Taka, Ai Muramatsu, Youichiro Hattori, Hiroyuki Hirate, Toshimasa Toyo’oka, Kazuya Sobue
  • Journal Title: Journal of Intensive Care Volume: 1 Issue: 1 Pages: 15-15
  • DOI: 10.1186/2052-0492-1-15
  • Peer Reviewed
• [Journal Article] Age-related requisite concentration of sevoflurane for adequate sedation with combined epidural-general anesthesia (2013)
  • Author(s): Y Fujita, A Kondo, H Yamauchi, E Kako, K Sobue
  • Journal Title: Korean J Anesthesiol Volume: 64 Issue: 6 Pages: 489-93
  • DOI: 10.4097/kjae.2013.64.6.489
  • Peer Reviewed
• [Journal Article] Development of a stable isotope dilution UPLC-MS/MS method for quantification of dexmedetomidine in a small amount of human plasma (2013)
  • Author(s): Inoue K, Sakamoto T, Fujita Y, Yoshizawa S, Tomita M, Min JZ, Todoroki K, Sobue K, Toyo'oka T
  • Journal Title: Biomed Chromatogr Volume: 27 Issue: 7 Pages: 853-8
  • DOI: 10.1002/bmc.2870
  • Peer Reviewed
• [Journal Article] Latent myasthenia gravis revealed by protracted postoperative effect of non-depolarizing neuromuscular blockade. (2012)
  • Author(s): S Ito, Y Fujita, H Sasano, K Sobue.
  • Journal Title: Journal of Anesthesia Volume: 26 Issue: 6 Pages: 953-4
  • DOI: 10.1007/s00540-012-1432-4
  • NAID: 10031147442
  • Peer Reviewed
• [Presentation] チェックリストを用いたブリーフィングの導入と、インシデント、アクシデントに与える影響 (2015)
  • Author(s): 藤田義人、宮本 泰周、田中 美緒、中村 絵美、住江 百合、椋田 崇、森 由紀子、兒玉 絵里、安田 吉孝、安藤 一雄、吉野 博子、赤堀 貴彦、橋本 篤、黒川 修二、神立 延久、佐藤 祐子、下村 毅、畠山 登、木下 浩之、藤原 祥裕
  • Organizer: 第23回日本集中治療医学会東海北陸地方会 総会・学術集会
  • Place of Presentation: 福井
  • Year and Date: 2015-06-20
• [Presentation] Discrepancy of correlation for dosage of long-term administration of dexmedetomidine and plasma concentrations between adults and infants (< 10 kg) in critically ill patients (2015)
  • Author(s): Fujita Y., Inoue K., Yoshizawa S., Sakamoto T., Toyo'oka T., Sobue K
  • Organizer: Annual Meeting of the European Anesthesiology
  • Place of Presentation: Berlin
  • Year and Date: 2015-05-30 – 2015-06-02
• [Presentation] 小児ICUでの瞳孔記録計(NPi-100)の有効性調査 (2015)
  • Author(s): 上村友二、播磨恵、仙頭佳起、宮津光範、太田晴子、徐民恵、森島徹朗、平手博之、藤田義人、祖父江和哉
  • Organizer: 日本集中治療医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2015-02-09 – 2015-02-11
• [Presentation] 当院ICUにおける誤薬防止のための多角的な対策 ～セーフラベルシステムの導入と多種職への教育の効果～ (2015)
  • Author(s): 志田恭子、徐民恵、森島徹朗、平手博之、草間宣好、杉浦健之、藤田義人、田村哲也、岡野将典、祖父江和哉
  • Organizer: 日本集中治療医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2015-02-09 – 2015-02-11
• [Presentation] 名古屋市立大学病院におけるRapid Response System運用の工夫と課題 (2015)
  • Author(s): 佐野文昭、藤田義人、草間宣好、杉浦健之、岡野将典、辻達也、小笠原治、大内田絵美、伊藤秀和、祖父江和哉
  • Organizer: 日本集中治療医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2015-02-09 – 2015-02-11
• [Presentation] 本学クリニカルクラークシップにおける麻酔科プログラムの自己分析と課題 (2014)
  • Author(s): 祖父江和哉、徐民恵、宮津光範、太田晴子、平手博之、杉浦健之、藤田義人
  • Organizer: 日本臨床麻酔学会
  • Place of Presentation: 東京
  • Year and Date: 2014-11-01 – 2014-11-03
• [Presentation] 殿皮神経障害が原因と考えられた腰痛の1症例 (2014)
  • Author(s): 草間宣好、藤田義人、浅井明倫、冨田麻衣子、播磨恵、竹内直子、幸村英文、平手博之、祖父江和哉
  • Organizer: 日本ペインクリニック学会
  • Place of Presentation: 東京
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] A型ボツリヌス毒素容量調節に難渋し中和抗体価測定を行った眼瞼痙攣の1例 (2014)
  • Author(s): 太田晴子、杉浦健之、大堀久、幸村英文、平手博之、草間宣好、藤田義人、冨田麻衣子、吉澤佐也、祖父江和哉
  • Organizer: 日本ペインクリニック学会
  • Place of Presentation: 東京
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] 当院ICUにおける誤薬・誤投予防止の取り組み－セーフラベルシステムの導入－ (2014)
  • Author(s): 志田恭子、前田純依、宮津光範、竹内直子、幸村英文、平手博之、草間宣好、杉浦健之、有馬一、藤田義人、祖父江和哉
  • Organizer: 第22回日本集中治療医学会東海北陸地方会 総会・学術集会
  • Place of Presentation: 愛知
  • Year and Date: 2014-06-14
• [Presentation] 帝王切開手術時の母体へ糖質輸液が母体・新生児血糖値に及ぼす影響 (2014)
  • Author(s): 吉澤佐也、藤田義人、冨田麻衣子、藤掛数馬、播磨恵、祖父江和哉
  • Organizer: 日本麻酔科学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] エリスロポエチン投与によるミクログリアを介した脳保護効果 (2014)
  • Author(s): 田村哲也、青山峰芳、藤田義人、仲野実輝、浅井清文、祖父江和哉
  • Organizer: 日本麻酔科学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] 重度低ナトリウム血症を来した帯状疱疹の1症例 (2014)
  • Author(s): 浅井明倫、杉浦健之、冨田麻衣子、吉澤佐也、太田晴子、徐民恵、草間宣好、平手博之、藤田義人、祖父江和哉
  • Organizer: 東海・北陸ペインクリニック学会第25回東海地方会
  • Place of Presentation: 愛知
  • Year and Date: 2014-05-10
• [Presentation] エリスロポエチンによる脳保護効果とミクログリアの活性 (2014)
  • Author(s): 田村哲也、太田晴子、藤田義人、祖父江和哉
  • Organizer: 日本神経麻酔・集中治療研究会
  • Place of Presentation: 沖縄
  • Year and Date: 2014-04-18 – 2014-04-19
• [Presentation] 当院におけるRapid Response System導入時の工夫 (2013)
  • Author(s): 西澤義之、藤田義人、幸村英文、平手博之、服部友紀、杉浦健之、有馬一、笹野寛、大内田絵美、祖父江和哉
  • Organizer: 日本集中治療医学会学術集会
  • Place of Presentation: 松本