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Mechanism of ovarian carcinogenesis due to 26S proteasome activation by protooncogene gankyrin mononeddylation

Research Project

Project/Area Number 24592514
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKyoto University

Principal Investigator

HIGASHITSUJI Hisako  京都大学, 医学(系)研究科(研究院), 助教 (20402852)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsgankyrin / mononeddylation / S6 ATPase / 26S proteasome / polyubiquitylation / synthetic biology / mdm2 / p53 / NEDD8 / E3 ubiquitin ligase / pRB / 卵巣がん / ガンキリン / プロテアソーム / 卵巣癌 / 癌遺伝子
Outline of Final Research Achievements

In ovarian cancers, gankyrin and Nedd8 are overexpressed. In vitro gankyrin neddylation assay (bacteria-expressed and purified E1, E2, Mdm2, Nedd8, gankyrin, ATP) produces gankyrin mononeddylation, that is verified by western blot of anti-gankyrin antibody and anti-Nedd8 antibody. Due to synthetic biological method, polycistronic affinity-tagged gankyrin, Nedd8, E1, E2, and Mdm2 are expressed into Rosetta bacterial cells. Gankyrin mononeddylation occurs. This expressed and purified mononeddylated gankyrin enhances p53 and pRB polyubiquitylation and 26S proteasome-mediated protein degradation. This effect is enhanced by full-length S6 ATPase co-expression. However it is suppressed by C-terminal truncated S6 ATPase mutant.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (9 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results)

  • [Journal Article] Stress response protein cirp links inflammation and tumorigenesis in colitis-associated cancer.2014

    • Author(s)
      Sakurai T, Kashida H, Watanabe T, Hagiwara S, Mizushima T, Iijima H, Nishida N, Higashitsuji H, Fujita J, Kudo M.
    • Journal Title

      Cancer Res.

      Volume: 74 Pages: 6119-28

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Overexpression of gankyrin in mouse hepatocytes induces hemangioma by suppressing factor inhibiting hypoxia-inducible factor-1 (FIH-1) and activating hypoxia-inducible factor-1.2014

    • Author(s)
      Liu Y, Higashitsuji H, Higashitsuji H, Itoh K, Sakurai T, Koike K, Hirota K, Fukumoto M, Fujita J.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 432(1) Pages: 22-27

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hypothermia protects against fulminant hepatitis in mice by reducing reactive oxygen species production.2013

    • Author(s)
      Sakurai T, Kudo M, Watanabe T, Itoh K, Higashitsuji H, Arizumi T, Inoue T, Hagiwara S, Ueshima K, Nishida N, Fukumoto M, Fujita J.
    • Journal Title

      Dig Dis

      Volume: 31 Issue: 5-6 Pages: 440-6

    • DOI

      10.1159/000355242

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel enhancer that binds Sp1 and contributes to induction of cold-inducible RNA-binding protein (cirp) expression in mammalian cells.2012

    • Author(s)
      Sumitomo Y, Higashitsuji H, Higashitsuji H, Liu Y, Fujita T, Sakurai T, et al
    • Journal Title

      BMC Biotechnol

      Volume: 12 Issue: 1 Pages: 72-72

    • DOI

      10.1186/1472-6750-12-72

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cold-inducible RNA-binding protein (Cirp) interacts with Dyrk1b/Mirk and promotes proliferation of immature male germ cells in mice2012

    • Author(s)
      Masuda T, et al.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 109 Issue: 27 Pages: 10885-108901

    • DOI

      10.1073/pnas.1121524109

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ほ乳類低温ショックタンパク質CIRPはBRCA1による相同組み替えによるDNA修復を促進する2014

    • Author(s)
      東辻久子
    • Organizer
      第69回日本消化器外科学会総会
    • Place of Presentation
      郡山市立文化センター
    • Year and Date
      2014-07-16 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] ほ乳類低温ショックタンパク質Cirpは皮膚創傷治癒を促進する2013

    • Author(s)
      藤田潤、東辻久子、東辻宏明
    • Organizer
      第8回臨床ストレス応答学会
    • Place of Presentation
      松本市、信州大学医学部付属病院、大会議室
    • Related Report
      2013 Research-status Report
  • [Presentation] Overexpression of gankyrin in mouse hepatocytes induces hemangioma.2013

    • Author(s)
      東辻宏明、東辻久子、藤田潤
    • Organizer
      第21回JDDW2013
    • Place of Presentation
      東京都品川区
    • Related Report
      2013 Research-status Report
  • [Presentation] ACC1脂質代謝系酵素によるBRCA1のhomologous recombination の調節機序2013

    • Author(s)
      東辻宏明、東辻久子
    • Organizer
      日本消化器病学会
    • Place of Presentation
      鹿児島市
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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