| Project/Area Number |
24592687
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Fukuoka University |
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Principal Investigator |
SASSA, Yukio 福岡大学, 医学部, 講師 (80580315)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Shigeo 九州大学, 医学部, 講師 (50363370)
ISHIBASHI Tatsuro 九州大学, 医学部, 教授 (30150428)
FUKUSHIMA Syuuichiro 大阪大学, 基礎工学部, 助教 (40362644)
YASUI Takeshi 徳島大学, 工学部, 教授 (70314408)
KONO Tosihiro 福岡大学, 医学部, 教授 (40117106)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 眼増殖性疾患 / 網膜色素上皮細胞 / SHG光 / コラーゲン / リモデリング / SHG顕微鏡 / ペリオスチン / テナシンC / 牽引性網膜剥離 / 増殖糖尿病網膜症 / 増殖性網膜症 / コラーゲン可視化 / テナシンＣ / コラーゲンリモデリング / レーザー脈絡膜新生血管モデル / 走査電子顕微鏡 / 線維化 |
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| Outline of Final Research Achievements |
We recently have revealed the ocular proliferation-associated genes by microarray analysis of intraocular proliferative tissues. We have previously reported a new realtime collagen-remodeling models using the SHG microscope. SHG microscope is based on a nonlinear optical effect known as second-harmonic generation and can image collagen structures without staining. In this project, we use the multi-well culture slides(μ-Slide Angiogenesis, ibidi) to evaluate the effect of multi proteins on collagen remodeling at once. We evaluated the effect of occular proliferation-associated proteins including periostin and tenacin C on the collagen remodeling. We also revealed that periostin and tenacin C promoted the retinal cell(retinal endothelial cell and retinal pigment epithelial cell) migration, proliferation and tube-formation.
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