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Osx regulates osteoblast differentiation through association with coactivators

Research Project

Project/Area Number 24592784
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionTsurumi University

Principal Investigator

NAKASHIMA Kazuhisa  鶴見大学, 歯学部, 非常勤講師 (90252692)

Co-Investigator(Kenkyū-buntansha) AKIRA Nifuji  鶴見大学, 歯学部, 教授 (00240747)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords骨芽細胞 / 転写因子 / 細胞分化 / 分化
Outline of Final Research Achievements

Osteoblast differentiation is a multistep pathway in which multipotential mesenchymal cells differentiate into osteoblasts. The transcription factor Osterix (Osx) regulates osteoblast differentiation and bone-specific gene expression. However, Osx expression is detected not only in osteogenic tissue but also in chondrogenic tissues, suggesting the presence of molecules required to control osteogenesis by Osx. In the current studies, we have analyzed possible Osx partner molecules Osx coactivator 1 (OSC1) and Osx coactivator 2 (OSC2). The results suggest that the two molecules associate with Osx as a coactivator during osteoblast differentiation. Expression of OSC1 is induced during osteoblast differentiation in vitro. OSC1 and OSC2 directly interact with Osx and promote Osx-dependent transcriptional activity, suggesting that OSCs act as a transcriptional coactivator for Osx. Our results provide new insights into a transcriptional regulation of osteoblast differentiation by Osx.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (30 results)

All 2015 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (24 results)

  • [Journal Article] H3K9MTase G9a is essential for the differentiation and growth of tenocytes in vitro2015

    • Author(s)
      Wada S, Ideno H, Shimada A, Kamiunten T, Nakamura Y, Nakashima K, Kimura H, Shinkai Y, Tachibana M, Nifuji A.
    • Journal Title

      Histochem Cell Biol.

      Volume: in press Issue: 1 Pages: 13-20

    • DOI

      10.1007/s00418-015-1318-2

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Coordinated expression of H3K9 histone methyltransferases during tooth development in mice2015

    • Author(s)
      Kamiunten T, Ideno H, Shimada A, Nakamura Y, Kimura H, Nakashima K, Nifuji A.
    • Journal Title

      Histochem Cell Biol.

      Volume: 143 Issue: 3 Pages: 259-266

    • DOI

      10.1007/s00418-014-1284-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient expansion of mouse primary tenocytes using a novel collagen gel culture method2014

    • Author(s)
      Akemi Shimada, Satoshi Wada, Kouji Inoue, Hisashi Ideno, Taichi Kamiunten, Koichiro Komatsu, Akira Kudo, Yoshiki Nakamura,et al
    • Journal Title

      Histochem Cell Biol

      Volume: (in press) Issue: 2 Pages: 205-215

    • DOI

      10.1007/s00418-014-1191-4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Alendronate promotes bone formation by inhibiting protein prenylation in osteoblasts in rat tooth replantation model2013

    • Author(s)
      Komatsu K, Shimada A, Shibata T, Wada S, Ideno H, Nakashima K, Amizuka N, Noda M, Nifuji A
    • Journal Title

      J Endocrinol.

      Volume: 219 Pages: 145-158

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Predominant expression of H3K9 methyltransferases in prehypertrophic and hypertrophic chondrocytes during mouse growth plate cartilage development2013

    • Author(s)
      Ideno H, Shimada A, Imaizumi K, Kimura H, Abe M, Nakashima K, and Nifuji A
    • Journal Title

      Gene Expr Pat

      Volume: 13 Issue: 3-4 Pages: 84-90

    • DOI

      10.1016/j.gep.2013.01.002

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Improved methods for detection of ss-galactosidase (lacZ) activity in hard tissue2012

    • Author(s)
      Shimada A, Komatsu K, Nakashima K, Poschl E, Nifuji A
    • Journal Title

      Histochemistry and Cell Biology

      Volume: 137 Issue: 6 Pages: 841-847

    • DOI

      10.1007/s00418-012-0936-1

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Annexin A5欠損マウスは咀嚼筋肥大、歯の咬耗、口腔顔面の骨肥大を呈する2014

    • Author(s)
      島田明美、和田悟史、出野尚、上運天太一、小松浩一郎、工藤明、中村芳樹、中島和久、二藤彰
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜パシフィコ(神奈川県横浜市)
    • Year and Date
      2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 歯の発生分化におけるヒストンメチル化酵素の発現と機能2014

    • Author(s)
      上運天太一、出野尚、島田明美、中島和久、中村芳樹、二藤彰
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜パシフィコ(神奈川県横浜市)
    • Year and Date
      2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒストンメチル化酵素G9aによる間葉系細胞の増殖と分化の調節2014

    • Author(s)
      出野尚、島田明美、上運天太一、和田悟史、小松浩一郎、中村芳樹、安倍真澄、中島和久、二藤彰
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 腱細胞の発生分化におけるヒストンメチル化酵素G9aの機能2014

    • Author(s)
      和田悟史、出野尚、島田明美、上運天太一、中島和久、中村芳樹、二藤彰
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 歯の発生分化におけるヒストンメチル化酵素の発現と機能2014

    • Author(s)
      上運天太一、出野尚、島田明美、中島和久、中村芳樹、二藤彰
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 効率的で安定的なマウス腱細胞の初代培養法2014

    • Author(s)
      島田明美、和田悟史、出野尚、上運天太一、小松浩一郎、工藤明、中村芳樹、中島和久、二藤彰
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒストンメチル化酵素G9aによる腱細胞の分化制御2014

    • Author(s)
      和田悟史、島田明美、出野尚、立花誠、中村芳樹、中島和久、二藤彰
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒストン3リジン9メチル化酵素群(G9a, Glp, Suv39h1, Prdm2)のマウス歯牙発生における発現と機能2014

    • Author(s)
      上運天太一、島田明美、出野尚、中村芳樹、木村宏、立花誠、中島和久、二藤彰
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-07-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 間葉系細胞の増殖・分化過程におけるヒストンメチル化酵素G9aの機能2014

    • Author(s)
      出野尚、島田明美、上運天太一、和田悟史、小松浩一郎、荒木良子、立花誠、中村芳樹、安倍真澄、中島和久、二藤彰
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-07-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Three Histone 3 Lysine 9 Methyltransferases, G9a, GLP, And SETDB1, Are Expresssed During Mouse Tooth Development2013

    • Author(s)
      Kamiunten T, Shimada A, Ideno H, Nakashima K, Nakamura Y, Nifuji A.
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      Kobe International Conference Center and Kobe Portopia Hotel,
    • Related Report
      2013 Research-status Report
  • [Presentation] Predominant Expression of Histone 3 Lysine 9 Methyltransferases In The Prehypertrophc Chondrocytes During The Growth Plate Chondrocyte Development2013

    • Author(s)
      Ideno H, Shimada A, Kamiunten T, Imaizumi K, Nakashima K, Nifuji A
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      Kobe International Conference Center and Kobe Portopia Hotel,
    • Related Report
      2013 Research-status Report
  • [Presentation] enocytes Regulate Cell Survical of Osteoblasts In Vitro2013

    • Author(s)
      Wada S, Shimada A, Ideno H, Nakamura Y, Nakashima K, Nifuji A.
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      Kobe International Conference Center and Kobe Portopia Hotel,
    • Related Report
      2013 Research-status Report
  • [Presentation] Endocytic Incorporation of Alendronate Promotes Bone Formation2013

    • Author(s)
      Komatsu K, Shimada A, Shibata T, Wada S, Ideno H, Nakashima K, Nifuji A
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      Kobe International Conference Center and Kobe Portopia Hotel,
    • Related Report
      2013 Research-status Report
  • [Presentation] 三次元培養によ る培養腱細胞(テノサイト)単離法の確立2013

    • Author(s)
      島田明美、和田悟史、小松浩一郎、中島和 久、二藤彰
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山コンベンションセンター( 岡山県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 発生の 骨格組織分化におけるH3K9メチル 化酵素群の発現局在2013

    • Author(s)
      二藤彰、島田明美、中島和久
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山コンベンションセンター( 岡山県)
    • Related Report
      2013 Research-status Report
  • [Presentation] アレ ンドロネート直接作用による骨芽細胞分化の制御2013

    • Author(s)
      小松浩一郎、島田明美、柴田達也、中 島和久、網塚憲生、二藤彰
    • Organizer
      第55回歯科基礎医学会学術大 会
    • Place of Presentation
      岡山コンベンションセンター( 岡山県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 腱細胞による骨芽 細胞の制御2013

    • Author(s)
      和田悟史、島田明美、中村芳樹、中島 和久、二藤彰
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山コンベンションセンター( 岡山県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Annexin a5に よる骨と腱・靭帯付着部( enthesis)形成の調節2013

    • Author(s)
      島田明美、新井嘉則、出野尚、上運天太 一、中島和久、小松浩一郎、山 下照仁、江面陽一、網塚憲生、高橋直之、Ernst Pöschl, 二藤彰
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場( 兵庫県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 代謝産物の血液から唾液への移行についての検討2013

    • Author(s)
      柴田達也、杉本昌弘、金子未来、島田明美、 小松浩一郎、出野 尚、中島 和久、冨田 勝、二藤 彰
    • Organizer
      第 36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場( 兵庫県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Histone 3 lysine 9 methyltransferases, G9a, GLP, SUV39h, and PRDM2, are expresssed during mouse tooth development2013

    • Author(s)
      上運天太一、出野尚、島田明美、中村 芳樹、中島和久、木村宏、二藤 彰
    • Organizer
      第 36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場( 兵庫県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 靭帯腱付着部位における骨芽細胞の制御2013

    • Author(s)
      和田悟史、島田明美、出野尚、中村芳 樹、上運天太一、中島和久、二藤 彰
    • Organizer
      第36回 日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場( 兵庫県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Histone 3 lysine 9 methyltransferases G9a, GLP and SETDB1 are predominantly expressed in the prehypertrophc chondrocytes during the growth plate chondrocyte2012

    • Author(s)
      Hisashi Ideno, Akemi Shimada, Taichi Kamiunten, Kazuhiko Imaizumi, Hiroshi Kimura, Kazuhisa Nakashima, Akira Nifuji
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      博多、福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Soluble factors may mediate signals from tendons to bone2012

    • Author(s)
      Satoshi Wada, Akemi Shimada, Hisashi Ideno, Yoshiki Nakamura, Kazuhisa Nakashima, Akira Nifuji
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      博多、福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Expression of three histone 3 lysine 9 methyltransferases during development of mouse molar2012

    • Author(s)
      Taichi Kamiunten, Akemi Shimada, Hisashi Ideno, Yoshiki Nakamura, Hiroshi Kimura, Kazuhisa Nakashima, Akira Nifuji
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      博多、福岡
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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