Involvoment of satellite glial cells in trigeminal ganglion in tongue pain

• Project/Area Number: 24593064
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Nihon University
• Principal Investigator: SHINODA Masamichi 日本大学, 歯学部, 准教授 (20362238)
• Co-Investigator(Kenkyū-buntansha): IWATA Koichi 日本大学, 歯学部, 教授 (60160115) ; ASANO Masatake 日本大学, 歯学部, 准教授 (10231896) ; TANABE Natsuko 日本大学, 歯学部, 准教授 (10409097)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Ikuko 日本大学, 歯学部, 助手 (60459906) ; HONDA Kuniya 日本大学, 歯学部・ポスト・ドクトラル, フェロー (20548945) ; OMAGARI Daisuke 日本大学, 歯学部, 専修研究員 (10608699)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 舌痛症 / TRPV1 / Artemin / 熱痛覚過敏 / satellite cell / GAP43 / GFAP / TLR4 / GFRalpha3

Outline of Final Research Achievements

Burning mouth syndrome is characterized by altered sensory qualities, namely tongue pain hypersensitivity. We found that the mRNA expression of Artemin (Artn) in the tongue mucosa of burning mouth syndrome patients was significantly higher than that of control subjects, and we developed a mouse model of burning mouth syndrome by application of 2,4,6-trinitrobenzene sulfonic acid (TNBS) to the dorsum of the tongue. TNBS application to the tongue induced persistent, week-long non-inflammatory tongue pain and a significant increase in Artn expression in the tongue mucosa and marked tongue heat hyperalgesia whichwas inhibited by local administration of neutralizing anti-Artn antibody. These results suggest that the overexpression of Artn in the TNBS-treated tongue increases the membrane excitability of TG neurons innervating the tongue by increasing TRPV1 sensitivity, which causes heat hyperalgesia.

Research Products

• [Journal Article] Basic research and clinical investigations of the neural basis of orofacial pain (2015)
  • Author(s): Matsuka Y, Iwata K, Terayama R, Imamura Y, Maruhama K, Shinoda M, Tsuboi Y, Kondo M, Honda K, Katagiri A, Sugimoto T
  • Journal Title: J Oral Biosci Volume: 57 Issue: 1 Pages: 27-36
  • DOI: 10.1016/j.job.2014.09.001
  • NAID: 120006493387
  • Peer Reviewed / Open Access
• [Journal Article] Neural Communication in Trigeminal ganglion Contributes Ectopic Orofacial Pain (2013)
  • Author(s): Shinoda M, Iwata K.
  • Journal Title: J. Oral Biosci. Volume: 55 Issue: 4 Pages: 165-168
  • DOI: 10.1016/j.job.2013.06.003
  • Peer Reviewed
• [Presentation] Contribution of Artemin signaling in tongue trigeminal sensory neurons to non-inflammatory tongue pain (2014)
  • Author(s): Shionda M, Katagiri A, Honda K, Iwata K.
  • Organizer: The 15th world congress on pain
  • Place of Presentation: Buenos Aires, Argentine Republic
  • Year and Date: 2014-10-06 – 2014-10-11
• [Presentation] Artemin signaling contributes to the tongue pain in burning mouth syndrome mice. (2013)
  • Author(s): Shionda M, Katagiri A, Honda K, Iwata K.
  • Organizer: The 4th International Congress on Neuropathic Pain.
  • Place of Presentation: Toronto Canada
• [Presentation] 舌痛症におけるArteminの役割 (2013)
  • Author(s): 篠田雅路
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山コンベンションセンター （岡山市）
  • Invited
• [Presentation] 顎顔面口腔領域における異所性痛覚過敏の末梢神経機構 (2013)
  • Author(s): 篠田雅路
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山コンベンションセンター （岡山市）
  • Invited
• [Presentation] 顎顔面領域の異常疼痛発症におけるマイクログリア―ニューロン連関
  • Author(s): 篠田雅路
  • Organizer: 第17回日本口腔顔面痛学会
  • Place of Presentation: 慶応大学（東京）
  • Invited
• [Presentation] Altered artemin signaling in tongue trigeminal sensory neurons contributes to TNBS-induced tongue pain
  • Author(s): Shinoda M 他
  • Organizer: The 14th World Congress on Pain
  • Place of Presentation: ミラノ（イタリア）
• [Presentation] マウス舌におけるTNBS誘発熱痛覚過敏に対するArteminの役割
  • Author(s): 篠田雅路 他
  • Organizer: 第３４回日本疼痛学会
  • Place of Presentation: 熊本市国際交流会館（熊本）
• [Presentation] Involvement of Artemin and GFRa3 on nociception in rat model of burning mouth syndrome
  • Author(s): Shinoda M 他
  • Organizer: 8th FENS Forum of neuroscience
  • Place of Presentation: バルセロナ（スペイン）
• [Book] 疼痛研究の最前線 ファインケミカル (2014)
  • Author(s): 岩田幸一、富永真琴、篠田雅路、古江秀昌、歌大介、秋元望、柿木隆介、井関雅子、山本隆充、川嶋文人、幾島賢治、幾島嘉浩、幾島将貴
  • Publisher: シーエムシー出版
• [Remarks] 生理学
  • URL: http://www.dent.nihon-u.ac.jp/phisio/gs_top.html