Development of low polar molecules-targetted Live single-cell MS by piezo-oscillated nano-CI.
Project/Area Number |
24619004
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
オミクス計測科学
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Research Institution | Fukushima Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
MIZUNO HAJIME 理化学研究所, 生命システム研究センター, 研究員 (30457288)
MASUJIMA TSUTOMU 理化学研究所, 生命システム研究センター, グループリーダー (10136054)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 質量分析 / メタボロミクス / ナノスプレー / 化学イオン化 / 一細胞分析 / 1細胞分析 |
Outline of Final Research Achievements |
We developed Live single-cell MS method which allows serial analysis from single cell content recovery to direct ionization of included molecules extracted with ionization solvent using a nanospray chip followed by mass spectrometric measurement. In order to apply this method for low-polar molecules, we made a nanospray-oscillation device using a piezo element to nebulize solvent. Ionization was achieved by a corona discharge device. We found that signal of low-polar molecules was increased whereas background signal of environmental origin was also increased. To improve background noise, we made a device for ionization in clean charcoal-treated nitrogen gas-environment. We confirmed that signals of low-polar molecules were increased compared to the original method.
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Report
(5 results)
Research Products
(20 results)
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[Journal Article] Increase in dicentric chromosome formation after a single CT scan in adults.2015
Author(s)
Abe Y, Miura T, Yoshida MA, Ujiie R, Kurosu Y, Kato N, Katafuchi A, Tsuyama N, Ohba T, Inamasu T, Shishido F, Noji H, Ogawa K, Yokouchi H, Kanazawa K, Ishida T, Muto S, Ohsugi J, Suzuki H, Ishikawa T, Kamiya K, Sakai A.
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Journal Title
Scinetific Reports
Volume: 9
Issue: 1
Pages: 13882-13882
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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