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Application of sensitive EMT/MET reporter to identification of target genes for breast cancer stem cells.

Research Project

Project/Area Number 24650620
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Tumor biology
Research InstitutionKyoto University

Principal Investigator

YOSHIKAWA Kiyotsugu  京都大学, 医学(系)研究科(研究院), 准教授 (40333562)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsEMT / 乳癌幹細胞 / MET / レポーター / 発現クローニング / トリプルネガティブ乳癌 / 上皮間葉転換 (EMT) / 間葉上皮転換 (MET) / 乳癌 / ライブラリースクリーニング
Research Abstract

In order to identify cDNA/shRNA that can induce EMT or MET, we established a reporter system where vimentin or E-cadherin promoter drives blasticidine-resistant gene and GFP in HMLE epithelial or MDA-MB-231 mesenchymal breast cancer cell line. The cells were infected with cDNA or shRNA library and subjected to blasticidine selection. In EMT screening, Snail and Twist2 were identified as EMT inducer, and two genes, members of mitochondrial complex I, and a hub gene in signal transduction were identified to support soft aga-colonogenecity of mesenchymal cells. In MET screen, a potent MET-inducing shRNA (called shP1) was identified. The shP1 was tested for soft agar colony formation using monoclonal dual reporter cell clones from SUM159 mesenchymal breast cancer cell line. Interestingly, there are two sets of reporter clones with different response to shP1. Colony formation did not decreased in one set of reporter clones, while in the other clone shP1 reduced colony formation.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (8 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] Sal-like 4 (SALL4) is the factor for cell dispersion in basal-like breast cancer2013

    • Author(s)
      Itou, J., Matsumoto,Y., Yoshikawa,Y.and Toi,M.
    • Journal Title

      FEBS Letters

      Volume: 587 Pages: 3115-3121

    • Related Report
      2013 Final Research Report
  • [Journal Article] Sal-like 4 (SALL4) is the factor for cell dispersion in basal-like breast cancer.2013

    • Author(s)
      Itou, J., Matsumoto,Y., Yoshikawa,Y.and Toi,M
    • Journal Title

      FEBS Letters

      Volume: 587 Pages: 3115-3115

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Application of sensitive EMT reporter to identification of mammary stem cell-related genes2013

    • Author(s)
      Yoshikawa,K., Matsumoto, Y., Ito, J., Shiojiri Y., Reinherz E., To M.
    • Organizer
      AACR tumor invasion and metastasis meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] EMT/METレポーターを用いた網羅的乳癌幹細胞標的遺伝子の同定の試み2013

    • Author(s)
      松本純明、伊東潤二、塩尻好恵、Ellis Reinherz,戸井雅和、吉川清次
    • Organizer
      第21回日本乳癌学会
    • Place of Presentation
      浜松
    • Related Report
      2013 Final Research Report
  • [Presentation] EMT/METレポーターを用いた網羅的乳癌幹細胞標的遺伝子の同定の試み2013

    • Author(s)
      松本純明、伊東潤二、塩尻好恵、Ellis Reinherz, 戸井雅和、吉川清次
    • Organizer
      第21回日本乳癌学会
    • Place of Presentation
      浜松
    • Related Report
      2013 Annual Research Report
  • [Presentation] Application of sensitive EMT reporter to identification of breast cancer stem cell-related genes.2013

    • Author(s)
      Kiyotsugu Yoshikawa
    • Organizer
      AACR meeting: tumor invasion and metastasis
    • Place of Presentation
      アメリカ・サンディエゴ
    • Related Report
      2012 Research-status Report
  • [Presentation] Application of sensitive epithelial-mesenchymal transition (EMT) reporter to identification of mammary stem cell-related genes2012

    • Author(s)
      Yoshikawa K.
    • Organizer
      OOTR 8^<th > anuual conference
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of sensitive EMT reporter to identification of mammary stem cell-related genes2012

    • Author(s)
      Yoshikawa,K., Matsumoto, Y., Ito, J., Shiojiri Y., Reinherz E., To M.
    • Organizer
      Gordon Research Conference, mammary gland biology
    • Place of Presentation
      Barga, Italy
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of sensitive EMT reporter to identification of mammary stem cell-related genes.2012

    • Author(s)
      Kiyotsugu Yoshikawa
    • Organizer
      Organisation for oncology and translational research 8th annual meeting
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Application of sensitive EMT reporter to identification of mammary stem cell-related genes.2012

    • Author(s)
      Kiyotsugu Yoshikawa
    • Organizer
      Gordon research conference
    • Place of Presentation
      イタリア・ピザ
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.dsk.med.kyoto-u.ac.jp/

    • Related Report
      2013 Final Research Report
  • [Remarks] 京都大学ー大日本住友製薬(DSK)連携プロジェクト悪性制御研究ラボ

    • URL

      http://www.dsk.med.kyoto-u.ac.jp/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 大日本住友製薬ー京都大学(DSK)連携プロジェクト悪性制御研究ラボ

    • URL

      http://www.dsk.med.kyoto-u.ac.jp/

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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