Estimation of an alternative biosynthetic pathway for co-factors by close investigation of genome databases.

Research Project

Project/Area Number	24651235
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Living organism molecular science
Research Institution	Hokkaido University
Principal Investigator	DAIRI Tohru 北海道大学, 大学院・工学研究院, 教授 (70264679)
Co-Investigator(Renkei-kenkyūsha)	SATOH Yasuharu 北海道大学, 大学院・工学研究院, 助教 (30360928)
Project Period (FY)	2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords	生合成 / 4-アミノ安息香酸 / グルタチオン / エルゴチオネイン / タウリン / 新規生合成経路
Research Abstract	The invovment of new enzymes for co-factors biosynthesis was examined. pABA; A function unknown gene, NE1434, was obtained from Nitrosomonas europaea by shotgun cloning experiment using pABA-auxotrophic E. coli mutant (△pabABC) as a host. Glutathione; Streptomyces strains do not use glutathione. However, SCO0910 was confirmed to be glutamate-cysteine ligase and was essential for ergothioneine biosynthesis. Taurine; SCO3035 in S. coelicolor A3(2) was reported to be the first enzyme in taurine biosynthesis (J. Bacteriol. 188, 5561, 2006). To investigate taurine biosynthesis in S. coelicolor A3(2), SCO3416, 2782, and 2017, putative enzymes catalyzing thesecond reaction, were used for in vitro assay. However, all enzymes did not show the expected activity.